Experimental Study On Influence Of Sanqing Glucose-lowering Compound Against Glucose Lipid Of Type 2 Diabetic Rats

Aims To observe Sanqing glucose-lowering compound's influence on the type 2 diabetic rats glucolipid metabolism and detect the index level of change of tumor necrosis factor TNF-a, insulin, lepton. we can clear that the curative effect of drug treatment which can provide theoretical basis for the further clinical application.Methods Seventy healthy male WISTAR rats weighing 180±20g are needed. From them we extract randomly ten feeded with ordinary feed as normal controls. The remaining sixty with four weeks'high-fat feed will have continuous two times'intraperitoneal injection mould with small dose (STZ). A week later, use blood glucose meter measuring fasting plasma glucose in the values 11.1 mmol/L as film standard. Then Based in fasting plasma glucose, with the methods of random area, the rats will be divided into five groups: model control group(N group), Sanqing low dose group(SD group), Sanqing medium dose group(SZ group), Sanqing high dose group(SG group), rosiglitazone group(L group). Ten rats for each group.Irrigating stomach for the rats: pure water for the normal group and model control group; Sanqing high dose group,Sanqing medium dose group and Sanqing low dose group of press 8.05g/kg,4.03 g/kg,1.61 g/kg served sanqing glucose-lowering compound respectively, infused with the equivalent of adult retrieved 10,5,2 times irrigation stomach, rosiglitazone group irrigation clothing rosiglitazone 0.29 mg/kg. Measuring fasting plasma glucose, plasma glucose of half an hour after meal and two hours after meal on the day before the drug time, the second weekend and the fourth weekend. The rats will have a weight measuring every week. To drug four weeks later, the abdominal aorta took blood serum, detection separation when blood lipid and liver and kidney function, including CHO,TG,HDL-C,LDL-C,ALT,AST,Cr,BUN. We used ELISA method to detect levels of serum TNF-α, radioimmunoassay method to detect levels of serum leptin and insulin.Results1. To drug 4 weeks later, model group compared with normal weight value control weight dropped significantly, visible statistically significant difference(P<0.01).2. Give medicine before and after 2 weeks for medicine, model between groups with normal fasting glucose statistical significant group compared visible difference(P<0.05). In addition to the normal group is compared between each other outside, fasting blood sugar found no statistical significant difference(P>0.05). Give medicine four weeks later, each group fasting blood sugar visible statistically significant difference(P<0.05), and SD group of fasting blood sugar level decreased obviously, not including pairwise comparison shows the normal group, SD group and M group visible statistics significant difference(P<0.05).3. Give medicine for medicine before and after two weeks, N group and each made module comparison, 2-hr plasma glucose are visible statistically significant difference(P<0.05). In addition to the normal group made module beyond comparison, 2-hr plasma glucose found no statistical difference(P<0.05). Give medicine four weeks later, each group 2-hr plasma glucose visible statistical significant difference(P<0.05), and SD group 2-hr plasma glucose levels decreased obviously, not including pairwise comparison shows the normal group, the SD group and SG, M, L group visible statistics significant difference(P<0.05).4. Give medicine four weeks later, each group CHO and TG statistical significant level visible difference(P<0.05), pairwise comparison shows SZ, SD, M group with CHO, reduced levels of normal control group visible statistically significant difference(P<0.05), SZ, SD group, the lower level with TG normal control group visible statistically significant difference(P<0.05).5. Give medicine four weeks later, each group of ALT, AST level found no statistical significant difference(P>0.05). Each model group and N group of serum BUN level is elevatory, visible statistics are significant difference(P<0.05), each group Cr level is not statistically significant difference(P>0.05).6. Give medicine four weeks later, each group leptin leels visible statistically significant difference(P<0.05), pairwise comparison shows SG, SZ,SD,L group compared with normal control group statistical significant difference is visible(P<0.05), each group insulin, TNF alpha level found no statistical.Conclusion1. Sanqing glucose-lowering compound reduced fasting glucose and blood glucose levels 2-hr of type 2 diabetic rats low dose group the effect is better than high, middle dose group of sage and rosiglitazone.2. We can't sure Sanqing glucose-lowering compound in type 2 diabetic compound lipid-lowering effects in rats.3. In this study, we didn't observe Sanqing glucose-lowering compound has protective effect on liver and renal function of type 2 diabetic rats.4. In this study,we didn't observe Sanqing glucose-lowering compound has influence on the serum levels of tumor necrosis factor, leptin, insulin levels of type 2 diabetic rats .