Enclosed Compound Dressing Scaffolds Preparation And Evaluation Of Stent

BackgroundAccording to statistics, the United States each year up to 150 million people of burn patients. In China, every year for a variety of trauma patients who require treatment of up to tens of millions. In present the function of wound healing using a wide range of dressings, such as passive dressings, interaction-based dressings, biological activity and natural nanometer-type dressings, but some of the roles of these dressings are not limited to, the price is too expensive. Therefore, inexpensive choice, whether in wound dressings or on the financial burden is crucial for wound healing.With the wound healing process in the pathophysiology of in-depth study of wound healing people more and more profound understanding of the process, which led to the continuous improvement of medical wound dressings and development. Dressing at home and abroad for medical research is limited to polymer materials and the combination of Western medicine, traditional Chinese medicine for the combination of polymer materials and wound dressings made of the research report no. Chinese herbal medicine used in acute trauma wounds of traditional medicine is a new advantage in the treatment of trauma, and herbs on drug resistance in the price and has an absolute advantage over western medicine. China's accession to WTO, traditional Chinese medicine to the world, therefore, combined with traditional Chinese medical dressing as the future direction of development of one of the dressing.Based on the research status and needs, we started studying a new type of Chinese medicine compound dressings, this dressing can play not only absorb exudate, protect the role of the wound, but also the interaction with the exudate, according to the amount of exudate to a slow Medicine contained in the release of stent dressing. And finished products made from inexpensive, highly efficient, applicable to all kinds of trauma patients, medical workers and patients to facilitate operation and use.ObjectiveIn incent years, wound dressing research has developed quickly, but few reports the closed dressings prepared by traditional Chinese medicine combined with polymer composite, the efficacy of existing wound dressing is limited and not suitable for all wounds. The issue is the specificity for a variety of traumatic and non-traditional waterproof dressings, isolation, and poor adhesion with the wound tissue and other shortcomings, developed a good water absorption, moisture retention, permeability and adhesion of the new type of Chinese medicine compound enclosed dressing stand,The dressings and dressings currently on the market compared with herbal medicine in China and other unique effect, and the price is cheap, non-toxic side effects and drug resistance. Various water-soluble high marks for the current market characteristics of the material,Selected medicinal properties of polymer materials with a set, At the same time dressing made of these polymer materials have good support but also water absorption, permeability, adhesion, etc. The best combination of raw materials and the best ratio has been selected. The prepared pre-loaded sponge dressing stand a good medicine solution development and test its performance. China's accession to WTO, traditional Chinese medicine to the world, therefore, combined with Chinese dressing one of the future direction of development.Methods1. Sponge dressing preparation and characterization of scaffolds:Firstly the chitosan, gelatin and polyvinyl alcohol were mixed, while the above materials were additionally mixed with sodium alginate. The scaffolds were prepared by mixing, precooling and freeze drying. The properties of the encolsed dressing scaffolds was evaluted by water absorption, moisture content, relative moisture retention, residues, adhesion and sensory index sorce, physical,chemical index score and comprehensive index score.2. Preparation of the drug Sponges and Release Investigation:Sponges will be obtained in solution with the integration of traditional Chinese medicine, the sea will be prepared to draw Chinese standard curve solution, observation of the prepared drug-loaded dressings Sea Sponges for 48 hours in vitro release methods and drugs in the electron microscope to download the ultrastructure of dressing sponge scaffold.3. Data analysis:Establishment of a database with Excel2007 Software, after the turn with the entry of data into SPSS data files, With the input data analysis software package with SPSS 13.0. Analysis of variance of factorial design is used; single effect analysis is conducted at different groups after fixed effect.When P is less than 0.05,it is considered to be statistically significant.Results1. Sponges prepared by the appearance of dressing case:Prepared in accordance with uniform design portfolio dressing stand 11, the first level group of the prepared 1,6-stent failed to gel, other groups have gel. Group 3, group 8, group 11 Sponges made of a high score in the sensory. They were 38 points,38 points, 39 points respectively. Group 3 Sponges for the appearance of color is milky white, opaque, good surface gloss, smooth surface, no bubbles to follow good skin, easy to get mortgage. Group 8 Sponges for the appearance of light yellow color, transparency, surface gloss, smooth surface, resistant to external forces without deformation, no bubbles, easy to get exposed. Section 11 support the appearance of yellow sponge dressings, translucency, surface gloss, smooth surface, no bubbles, follow the degree of skin is better, easier to obtain mortgage.2. Water absorption of the prepared dressing sponge and water content conditions: Different proportions of each group of water absorption and water content of the matrix formulation of the comparative overall significant difference between the groups, F=11.966,P=0.000å'ŒF=19.500, P=0.000,the outcome is considered to be significant.Further multiple comparisons using Dunnett T3. Water absorption of the multiple comparisons in each group showed that group 2 and 8 groups were significantly different (P=0.035), with the other groups no significant differences.3 groups,7 groups,11 groups with each group were no significant differences. (P>0.05).4 and 8 group,9 groups,10 groups were significantly different (P=0.011,P=0.031,P=0.016).5 groups with 8 groups,10 groups were significantly different (P=0.017,P=0.038). Water content of the multiple comparisons in each group showed that group 2, group 7 and other groups no significant difference (P<0.05). Group 3 and 4,8 group are different (P=0.027,P=0.024).4 group and 3 groups,8 groups,9 groups,10 groups,11 groups were significantly different (P=0.027,P=0.000,P=0.000, P=0.001,P=:0.004).5 groups with 8 group,9 groups,10 groups were significantly different (P=0.000, P=0.003,P=0.027).3. Sponges closed dressing recipe different proportions of matrix changes in the relative moisture:Matrix formula dressing made from different proportions relative moisture stent were significantly different F=4.275, P=0.002(P<0.05). Further comparison used Dunnett T3 method. Multiple comparisons between the groups was no significant difference (P>0.05)4. Sponges closed dressing recipe different proportions of matrix permeability changes:Each group for statistical analysis of the comparative permeability of the overall has no significant.,F=1.436 P=0.199,(P>0.05).Each group sponges scaffolds' ventilation rate fluctuated between 53% and 66%.The highest score of sponge scaffolds' ventilation is 2 group. The score is 9.09±1.80.5. Sponges closed dressing recipe different proportions of matrix residual stent and stent adhesion conditions change status:Matrix formula obtained by different proportions of the dressing support residues and statistically significant differen-ce F=4.309,P=0.002. Further comparison used Dunnett T3 method. Group 2 and group 3 was different significantly (P=0.035), Group 3 and group 7 was different significantly (P=0.040),4,5 groups,8 groups,9 groups,10 groups,11 groups with each group showed no significant difference (P>0.05).4 Sponges of the prepared dressing adhesion to stick a larger ball 17, ball diameter is 11.906mm.6. Physical and chemical index and comprehensive index of closed dressing score: Sealed sponge dressing percentage of the stent matrix formulation prepared Sponges different sensory index score, the highest score was 11 groups; Physical and chemical index scores were significantly different (F=6.552,P=0.000) Further use of LSD are re-comparison method, Group 2 and 8 groups,10 groups were significantly different (P=0.000,P=0.041).3 groups with 8 groups,9 groups, 10 groups were significantly different (P=0.000,P=0.020,P=0.011).4groups,5 groups,7 groups with 8 groups, have significant difference between each other (P <0.05). Compared with the other groups no difference(P>0.05).8 group and the other groups were significantly different (P<0.05); Composite indicator scores were significantly different (F=38.294,P=0.000), Further use of LSD are re-comparison method, Group 2,4 and 7 were not significant difference in addition to other groups with significant differences. Group 5 In addition to 9 groups,10 groups had no difference with the rest of the group differences.3 groups,8 groups and 11 groups were different with each groups(P>0.05)7. Sponges loaded closed dressing Ultrastructure and aperture:The scanning electron microscope the sample surface, bottom and longitudinal profile of the ultrastructure can very clearly see the surface and bottom bracket has a larger hole sizes within the pore structure, Longitudinal section on a more regular channel from the bottom up. Each of these holes and channels formed grid to form three-dimensional structure. SEM observations of the pore dressing Sponges, Up to 170μm, a minimum of 30μm, the average pore size of 100μm, Uniform pore structure, but there are a lot of holes inside the connected and the connection between the layers is thinner and more fibrous structure, resulting in the internal structure of the stent is rough.8. Closed drug delivery dressing Sponges performance:with traditional Chinese Medicine as a model drug, Sponges 48h samples showed a linear relationship of drug release, Chinese Sponges 60min-120min in the initial burst of drug release process there,2h after the slow release of drug Tends to a balance, and observed in this study has shown within 48 hours of the release of a stable state. Conclusion1. Dressing Sponges for building material substrate choice:access to a large number of documents and through trial and error many times, and ultimately determined by the chitosan matrix sponge dressings, gelatin, glycerin, polyethylene glycol and polyvinyl alcohol and so on.2. Dressing stent matrix preparation process, the gelatin into the temperature is 50℃～60℃in distilled water, while slowly adding, while stirring, in a water bath inside the time normally not more than 20min, and is equipped with active gelatin. Add cold distilled water and alcohol swelling should be 30min, then heated to 85℃-95℃, the time is generally 60min-120min. Chitosan should be added to the solution containing 2% acetic acid solution, placed in 60 oC water bath inside, time is about 60min before they can dissolve. Solution mixed with low-speed stirring using a magnetic stirrer.3. In this study, the prepared dressing sponge forming stents in 9 groups of indicators of sensory, physical and chemical indicators of integrated measurement, the best selection at the best volume ratio of matrix dressing: chitosan:gelatin:polyvinyl alcohol:glycerol:poly Glycol= 8.3:4.4:3.1:3.7:1.0.4. The scanning electron microscope the sample surface, bottom and longitudinal profile of the ultrastructure, you can very clearly see the surface and bottom bracket have holes of varying sizes, and there is a large range of pore structure on longitudinal section have a more the rules of the channel from the bottom up. Scanning electron microscopy dressing Sponges aperture, up to 170um, a minimum of 30μm, the average pore diameter of 99.75±45.18μm, uniform pore structure, but there are a lot of holes inside the connected and the connection between the layers is smaller and more like fiber structure, resulting in the internal structure of the stent is rough, is conducive to Chinese cell adhesion adhesion and growth of the wound.5. In this study, using Chinese medicine as a model drug, loaded in the prepared Sponges, medicine cream for the first 60min-120min have a burst of drug release process,2h after the slow release of drugs tends to a balance of 48 hours in the experimental observations, The drug release showed a good linear relationship, indicating that the function of slow-release stent with good.