| Objective:To explore the expression of peroxisome proliferator- activated receptor gamma (PPAR-γ) and T lymphocyte subsets in peripheral blood of children with asthmatic disease.Methods:Applying RT-PCR and flow cytometry (FCM) to measure the expression of PPAR-γand T lymphocyte subsets in children within acute asthma group, asthmatic pneumonia group and healthy control group.Correlation between PPAR-γand CD4~+, CD8~+T lymphocyte was analyzed.Results:Expression levels of PPAR-γin exacerbation asthma group was lower than asthmatic pneumonia group, and asthmatic pneumonia group was significantly lower than control group (P﹤0.01), pairwise comparison among the three groups were statistically significant (P﹤0.01). According to pathogenic results, asthmatic pneumonia patients were divided into RSV infection group, MP infection group, BOKA infection and mixed infection group. Compared with the control group, levels of PPAR-γin those four groups were statistically significant (P﹤0.01), but pairwise comparisons among the four groups got no significant difference (P﹥0.05). Percentage of CD3~+ lymphocytes of exacerbation asthma group and asthmatic pneumonia group were significantly lower than heathly control group (P﹤0.01), with on statistical variance found between exacerbation asthma group and asthmatic pneumonia group (P﹥0.05). Compared to heathly control group, percentage of CD4~+ lymphocytes in exacerbation asthma group and asthmatic pneumonia group were significantly higher (P﹤0.05), with significant differences between asthmatic pneumonia group and heathly control group. Compared with heathly control group, percentage of CD8~+ lymphocytes was significantly lower in exacerbation asthma group and asthmatic pneumonia group (P﹤0.05), with no significant difference between exacerbation asthma group and asthmatic pneumonia group (P﹥0.05). CD3-CD19~+and CD19~+CD23~+ lymphocytes of exacerbation asthma group and asthmatic pneumonia group were markedly increased than heathly control group (P﹤0.05), but variance of these two subsets in exacerbation asthma group and asthmatic pneumonia group was not statistically significance (P﹥0.05). PPAR-γand CD4~+expression was negatively correlated in exacerbation asthma group (r =-0.640, P﹤0.05), while CD8 ~+ expression was positively correlated (r=0.7030, P﹤0.01). In asthmatic pneumonia group PPAR-γand CD4 ~+ expression was negatively correlated (r=-0.667, P﹤0.05), with CD8~+ expression positively correlated (r = 0.7673, P﹤0.01).Conclusion:Levels of PPAR-γin peripheral blood decreased in children with asthma and asthmatic pneumonia; the development of PPAR-γagonist treatment of asthma may have some clinical application for treatment for patients with wheezing; cellular immunity disorder was involved in the pathogenesis in children with asthma and asthmatic pneumonia. |
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