Preparation And Antioxidant Activity Of Phenylethanoid Glycosides From Cistanche Salsa

Herba Cistanche, belonging to Orobanchaceae families, is a perennial high parasitic plants and also called Congrong, Dayun, Jinshun, Dijing. Herba Cistanche is widely distributed in the desert of Middle Asia, West Asia and Inner Mongolia, Sinkiang, Shanxi, Gansu, Ningxia and Qinghai provinces in China. Herba Cistanche is a famous traditional tonic medicine with kidney-replenishing and stimulating bowels evacuation activity. The active components from Herba Cistanche can meet different populations'need, and has a enormous market prospect. The aim of this paper is to investigate the extraction and antioxidant activity of phenylethanoid glycosides (PEG) from Cistanche salsa, and to provide theoretical basis for the further exploration of Cistanche salsa. The results are as follows:Showed by single factor experiment, the optimized condition of PEG extraction from Cistanche salsa was:extraction temperature 70℃, ethanol concentration 70%(v/v), ratio of solid to liquid 1:10, extraction time 2 h, extraction times 2.Through response surface optimizing experiment and regressing analysis, the optimum processing of extracting PEG from (Cistanche salsa) was obtained:ratio of solid to liquid 11.47, temperature 68.41℃, ethanol volume fraction 87.52%, time 2 h,2 extraction times. Under the condition, the concentration of PEG in the extraction solution was up to 47.82μg/mL.Microwave processing could significantly increase the yield of PEG, while the processing time of microwave and ultrasonic could not. The optimum microwave power was 450 W indicated by single factor experiment.Hexane-butanol was used to purify PEG in the experiment. However, it was complicated, dangerous and contaminative, compared to chromatography. Consequently, D101 macroporous absorption resin chromatography was chosen for the purification of PEGPEG possessed high antioxidant activity. The reducing power of PEG was lower than BHT, but was significantly higher compared to Vc at the concentration of 0.5 mg/mL. The DPPH radical scavenging ability was slightly lower than BHT and Vc, but was obviously higher compared to VE within the range of 2-60 mg/mL. Antilipid peroxidation ability of PEG was similar to BHT and was obviously higher than VE.