Protective Effects Of Soy Saponins On Acute Alcohol-induced Liver Damage

Chapter 1:The structure, classification and common bioactivity of soyasaponins are reviewed in details. The extraction, separation, purification and detection for soyasaponins are summarized. Then the alcoholic liver disease (ALD) and its pathogenesis are depicted in brief, as well as the treatment of ALD by natural plant chemicals. At the end, the backgrounds, significance, main content, and the innovative points of this assay are stated.Chapter 2:The high performance liquid chromatograph-mass spectrometry (HPLC-MS) is used for the identification of soyasaponins. Ten soyasaponins in the soy saponins are identified as follows:soyasaponins Aa, soyasaponins Ab, soyasaponins Ae, soyasaponins Bb, soyasaponins Bb', soyasaponins Bd, soyasaponins Be, soyasaponinsag, soyasaponinsβg, and soyasaponins (3a. In addition, the isoflavones in the soy saponins are determined by high performance liquid chromatograph (HPLC) and the result is as follows: daidzin (1.15 mg/g), glycitin (0.09 mg/g), genistin (2.68 mg/g), malonyldaidzin (1.24 mg/g), malonylgenistin (4.57 mg/g).Chapter 3:The protective effects of soy saponins (SS) against acute alcohol-induced hepatotoxicity were investigated in Institute of Cancer Research (ICR) mice. Administration of SS prior to alcohol significantly prevented the increases in mice serum aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), and lactate dehydrogenase (LDH) caused by alcohol, as well as hepatic triglyceride (TG), total cholesterol (TC), and malondialdehyde (MDA) levels. Mice treated with SS showed a better profile of the antioxidant system with normal superoxide dismutase (SOD), glutathione-S-transferase (GST), and glutathione peroxidase (GPx) activities, which were associated with the increase of hepatic glutathione (GSH) levels relative to the acute alcohol treated group. Supplement of SRE prevented alcohol-induced hepatic steatosis necrosis, inflammation and swelling, as indicated by liver histopathological studies. All these findings demonstrate that SS has protective effects on acute alcohol-induced liver damage.Chapter 4:The degradation kinetics of soyasaponin Bb is studied by classical isothermal kinetic method. Soyasaponin Bb is put into water bath (for 80℃,90℃,100℃treatment) and agitated reactor (for 110℃,120℃130℃treatment). The sampling process is carried out every 10 min and the content of soyasaponin Bb is determined by high performance liquid chromatograph-evaporative light-scattering detector (HPLC-ELSD). The degradation kinetics of soyasaponin Bb is in accord with the first order reaction by mathematical fitting. On this basis, the degradation activation energy (Ea) is calculated to be 31.71 KJ·mol-1 by the Arrhenius Equation.