| Objective:To observe the correlation between the intestinal permeability with damage severity and the level of E-cadherin protein after gut barrier injury in rats with intraperitoneal infection.Methods:Forty healthy adult Wistar rats were randomly divided into control group (celiotomy ones only) and groups of 12,24,36,48 hours post-operation of abdominal infection respectively (These groups were treated by cecal ligation and puncture to establish abdominal infection models). Each group contained 8 rats. After the rats were anaesthetized,2 cm of the ileum tissue,10cm from ileocecal junction was sampled for optical observations, including histological examination and evaluation of mucosal damage index. D-lactate in plasma was measured at 4 consecutive time points. The level of E-cadherin protein was detected using Western blot technique.Results:â‘ Intestinal barrier began to change significantly 12h after the infection, including higher mucosal damage index and higher level of D-lactate in plasma(P<0.01) than the control group. The damage reached the peak at 24h:the maximal mucosal injury score and the highest lever of D-lactate. Intestinal barrier began to restoration but remained still serious than the control group(P<0.01). D-lactate level had a significant correlation with mucosal damage index (r=0.489,P<0.01).â‘¡The level of E-cadherin protein was lower than that of control group(P<0.05). The expression of E-cadherin was significant different between the groups of 12h and 48h and that of 24h and 36h(P< 0.05). But no significant difference was detected beween the groups of 24h and 36h. D-lactate level had a significant correlation with the level of E-cadherin protein. (r=-0.605,P<0.01).Conclusion:Intestinal mucosal barrier was subjected serious damage and there were significant correlations between intestinal permeability with damage severity and the level of E-cadherin protein in rats with intraperitoneal infection. |
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