Studies On The Components And The Anti-tumor Activity Of Extract From Periploca Forrestii Schltr

Periploca forrestii Schltr belongs to the species of Asclepiadancae in Periploca Linn. It is mainly distributed in Southwest China. This plant can be used to promote expel wind-evil, blood circulation. This thesis made a systemic study on the components of Periploca forrestii Schlt.Five extracts of petroleum ether, chloroformethyl, ethyl acetate, n-butanol and water were obtained from ethanolic extract of the stalk bark of Periploca forrestil Schltr. Petroleum ether extract were analysed by gas chromatography-mass spectrometry(GC-MS)method. CompoundⅠ~Ⅶwere isolated respectively from chroloform extract by column chromatography of silica gel, Sephadex-LH-20 and semi preparative chromatography. The compounds were identified by NMR, MS and the physiccoche- mical constants. The activities of chemical components were identified by cell morphology.Ninety six compound were analysed from petroleum ether extract by gas chromatography-mass spectrometry(GC-MS)method. Seven components were obtained from chroloform extract of Periploca forrestii Schlt. CompoundⅠ~Ⅵdoesn't have anti-tumor activity. CompoundⅦhas anti-tumor activity but it has strong cytotoxicity to cells of K562.