| Object:The syndrome of Traditional Chinese Medicine is the summarization of pathogenesis in different stages of diseases. Since the same symptom has common clinical manifestation and pathogenesis, then they have common material foundation, which will probably reflect in the gene or protein. The essence in Kidney, coming from the reproduction of the parents, is the basis of embryonic development and identity with DNA in modrn medicine. In the case of the corresponding proteins express can the gene affect its biological functions. Proteomics is the study of protein expression and modification status, and protein-protein interactions under the overall level of living organisms or cells, leading to determine the function of human proteins and to clarify its function in the cell and then revealed the nature of living.Because the Kidney-yin deficiency and Kidney-yang deficiency is the basic syndrome of TCM, ancient physicians paid much more attentions on it. The Kidney-yin deficiency and Kidney-yang deficiency, which are the principal contradiction in a certain stage of a disease, must be influenced by the disease. The differences among varies diseases determined the differences among the same syndrome in varies diseases. Only when studied the differences and concluded the general rules can we analysis the Kidney-yin deficiency and Kidney-yang deficiency. As a result, "disease combined with syndrome" is an important ideas in studying the different proteins expression in the Kidney-yin deficiency and Kidney-yang deficiency.Using "disease combined with syndrome" in this study, we took Kidney-yin deficiency and Kidney-yang deficiency as the research object to screen the changes of cytokine through antibody chips research. We look for a major change of cytokines, to provide the basis for the TCM Kidney deficiency syndrome for objective indicators.Method:1 Using "disease combined with syndrome", selected the observers diagnosed with IgA nephropathy and subhealth condition, which are also in Kidney-yin deficiency and Kidney-yang deficiency, as the experimental group; choose healthy volunteers as the control group.2 Afters the sample collection and preparation, using direct labeling of biotin technique to detect the expression of multiple cytokines, and study the cytokine changes of Kidney-yin deficiency and Kidney-yang deficiency.Result:First,after the comparion between Kidney-yin deficiency group and normal control group, we received a total of 25 differentially expressed factors,2 of which are up(BMP5,TIMP4) and 23 are down(OSM,CXCR4,GCG,CXCR1,CTLA-4,Siglec-9,CCR7,IL-17A,IL-17F,TRADD,IGFBP-6,THBS2,IL-4R,HGFR,IL-RA,Osteoactivine,MMP10,M-CSFR,G-CSF R,CNTF,IL-17C,TGFβ2,FGF23). Classified by function, the differentially expressed factors mainly related to immune disorder, matabolism, protein biosynthesis, oxidative stress, damage repair, apoptosis, signal transduction and so on. The differentially expressed proteins in Kidney-yin deficiency conform to the theory of Kidney-yin deficiency of TCM, such as fever in chestpalms-soles, tidal fever and night sweating, lumbar debility, dizzy, susceptibility pericardium, listlessness, slow in reacting. Secondly, after the comparion between Kidney-yang deficiency group and normal control group, we received a total of 14 differentially expressed factors, all of which are up(Csk,BMP5,Frizzled1,Frizzled4,BDNF,CXCR3,LBP,LTBP1,IL-27RA,M-CSF,L-13RαⅡ,PECAM-1,Activin RⅡA,FGF R5). Classified by function, the differentially expressed factors mainly related to immune response, metabolism, apoptosis, cell adhesion, damage repair, oxidative stress reproductive capacity and etc. The differentially expressed proteins in Kidney-yang deficiency conform to the theory of Kidney-yang deficiency of TCM, such as lumbar debility, hair off and tooth loose, tinnitus and deafness, slow in reacting, brothers chills, hyposexuality, man's impotence and premature ejaculation, woman's infertility due to uterus cold.Thirdly, after the comparion between Kidney-yang deficiency group and Kidney-yin deficiency group, we received a total of 1 differentially expressed factors, of which is up(BMP5). Classified by function, the differentially expressed factors mainly related to apoptosis, bone synthesis and etc. TCM thought that Kidney stores essence, which is related to the body's growth, develepment and reproduction, and can compound the bone. The differentially expressed protein in Kidney-yang deficiency and Kidney-yin deficiency conforms to the theory of Kidney deficiency of TCM.Fourthly, TCM thought that the Kidney can store the essence, and is related to the body's growth, development and reproduction.The Kidney plays a main role in water metabolism and air lift, as well as the bone formation.The change of kindey essence is the cornerstone of the body's born,grow and old, which is also the base of yin and yang in the body. The differentially expressed protein in Kidney-yang deficiency and Kidney-yin deficiency are mainly related to immune function, metabolism, cell cycle, bone development, DNA repair, protein synthesis, reproductive capacity, neurotrophic, and other aspects of hematopoietic function, of which are consistent with the basic theory of TCM.Conclusion:Fistly, the differentially expressed protein in Kidney-yang deficiency and Kidney-yin deficiency are great and clearly different, which is conform to the theory of TCM. The differentially expressed protein can also provide scientific evidence to the TCM syndrom differentiation.Secondly, there are the same differentially expressed protein in Kidney-yang deficiency and Kidney-yin deficiency, which can provide scientific ideas and methods in studying the essence of the TCM. It also plays an important role in studying the mechanism of Kidney-yang deficiency and Kidney-yin deficiency.Thirdly, protein chip is an ideal method in the study of TCM-related protein. From the perspective of proteomics to study of Kidney-yang deficiency and Kidney-yin deficiency, we may reveal the essence of the kindey. |
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