To Study The Rabbit Semen Changes And Testicular Pathological Changes Before And After The Rabbit Bilateral Dyszoospermia Model Building

[Objective]The design of an improved artificial fake vagina collection of rabbit semen to collect rabbit semen analysis CASA. And then establish the bilateral dyszoospermia model in rabbits, take semen analysis and testicular pathological changes after three months. then drop the fixed tesis. three months later, semen analysis.study semen testicular pathological changes before and after the rabbit dyszoospermia.[Methods]1.Produced artificial fake vagina according to the characteristics of the Rabbit Corpus, then train rabbits and fake vagina semen.2.Raising the40California rabbits for four months, weighing3.5-4.5kg. and sexual maturity. Artificial fake vagina to collect semen.and semen analyzer in Spain SCA analysis software animal modules were detected and analyzed the semen.3.Testis tissue pathologic examination.observation of normal rabbit testicular tissue.4.Establish bilateral spermatogenic barriers model:using the urgical approach to the establishment of40healthy rabbit groin area in California dyszoospermia model.5.Feeding for three months after operation, then get testis tissue for pathologic examination, observe the pathological changes of the testicular tissue after operation.6. Artificial fake vagina to collect semen.and semen analyzer in Spain SCA analysis software animal modules were detected and analyzed the semen.7.Surgery20California rabbits with bilateral testicular descent and fixed in the scrotum (group A). the not decreased was the control group (group B).8.Feeding after three months, once again collected using artificial fake vagina semen SCA semen analysis to detect changes in semen quality and quantity of the observed, and A and B were analyzed and compared.[Results]1.Improved artificial fake vagina to take a fine convenient and simple, each taking a precision of0.5-2.0ml.2. The establishment of three months after the California rabbit semen volume of bilateral dyszoospermia model no significant changes (p>0.05). sperm density, vitality, live rates change significantly compared with preoperative.(P<0.01)3.Testicular descent fixed in the scrotum three months later, the California rabbit semen volume have a significant change (p>0.05) compared with the control group.sperm density, vitality, live rates than the control group significantly increased (p<0.05).4.Testicular descent fixed in the scrotum three months later, the California rabbit semen volume have a significant change (p>0.05) than those without surgery.sperm density, vitality, live rates than the control group significantly increased (p<0.05).5.Pathological changes in testicular tissue after surgery in March obvious decline in testicular histopathological changes than those without a fixed three months after the surgery, than the change did not decrease[Conclusions]1.the artificial fake vaginal Improved collect sperm convenience, the time sperm of sperm collection is more casual, and easy to semen collection, less pollution, easy to carry out semen analysis2.After surgery method to pull buried Rabbit bilateral testicular in the groin area to establish dyszoospermia model, the semen quality of rabbits decreased significantly.the amount oshowed no significant change3.Testicular descent and fixed, and compare with those Without surgeried. the semen quality of rabbits decreased, no significant change in amount. Compared with the control group, rabbit semen quality has been restored, the amount of no significant change.4.Compared with those without surgery, bilateral dyszoospermia model for three months later, the rabbit testicular histopathological change significantly.and have some recover when the Testicular descent and fixed three months later.