Study On Extraction And Seperation Process Of Maslinic Acid And Oleanolic Acid From Olea Europaea L. Pomace

The aim of this dissertation is to develop the extraction, separation andpurification technology of mainly effective components-Maslinic Acid(MA) andOleanolic Acid(OA)-from discarded pomace of Olea europare L. The processconditions and technology parameters which can be used for the industrializedproduction are obtained after the selection and optimization of extraction, separationand purification processes. The results have established a foundation for the use ofOlea europare L pomace effectively. The main contents of this dissertation include:1The main chemical compositions of Olea europare L(pomace) and the relevantextraction and separation technology research progresses of MA and OA arereviewed in this paper. In the meantime the application, benefits and limitations ofdifferent technologies are summarized. Then, we look to the development prospectand development direction of this research field.2It is investigated that the optimum ethanol extraction process conditions forthe MA and OA on the basis of single factor experiment. With the alcohol ratio (A),solid-liquid ratio (B) and reflux time (C) as investigation factors, the optimumethanol extraction process is determined by L9(34) orthogonal experimental design,which took extraction rate and total extracts yield of MA and OA as assessmentindices. It will obtain different extraction conditions to analyze the assessment indicesdepending on the different extraction purposes.3Using macroporous adsorption resins (MARs) as the key technology, L-9resin, which separates and purifies the MA and OA from discarded pomace of Oleaeuropare L well is obtained finally by studying the static adsorption and desorptionperformance of14kinds of MARs. Then the conditions of dynamicadsorption/desorption parameters on L-9resin are optimized.The desorption amountof MA and OA was7.79mg·g-1-1干树脂and10.75mg·g干树脂repectively at the bestcondition. Based on the results of the adsorption and desorption performances,L-9resin fits the pseudo-second order kinetic model well.The experiment proves that thetechnology of macroporous adsorption resin could give a dependable reference toseparate and purify effective components from discarded pomace of Olea europare Lfor industry production.4By silicagel column chromatography technology, MA and OA have beenisolated from ethyl acetate fraction of Olea europare L.pomace, then the preparation technology have been studied preliminary. The MA could be obtained from petroleumether-acetone10:1to5:1(v:v) gradient elute, and the purity could be further reached98%by recrystallization. The OA could be obtained from petroleum ether-acetone10:1to2:1(v:v) gradient elute, then isocratic elution with petroleum ether-acetone3:1(v: v), and the purity could be further reached98%by recrystallization.