Fat-soluble Chemical Constituents Of Lobelia

Lobelia sessilifolia Lamb is the root or the whole grass with root of Campanulacea.the study on active principles in the Lobelia sessilifolia Lamb aren't comprehensive and the study on pharmacology is just on the first step.Base of the previous research,I have got five compounds from Lobelia sessilifolia Lamb by using silica gel column chromatography, preparative thin-layer chromatography,reversed phase column chromatography,preparative HPLC.They are identified by EI-MS,¹HNMR,¹³CNMR.The two compounds were determined asβ-amyrin 3-O-β-palmitate,maniladiol 3-O-β-palmitate,Oleanolic acid,ursolic acid,stigmasterol.One of them was isolated from the genus Lobelia L.for the first time.To study the anti—invasive effects and its mechanisim and apoptosis induction of pentacyclic triterpenoid including ursolic acid and oleanolic acid in highly potentially metastatic lung cancer cell line.The Methods are invasive a—bilit Y,the adhesive ability, the migration ability and the activity of cathepsin B(CB)of PGCI 3 cells treated with the two drugs were determined by the invasion test or reconstituted basement membranc,the laminin adhesion test,the chemotactic migration test and the enzymologicaI method of CB.The apoptosis of the cells was detected with acridine orange ethidium bromide fluorescent s.tain(A()/EB)and TU-NEL.The result is All of the two drugs can inhibit the proliferative and invasive ability,and induce apoptosis of the PGCL3 human lung cancer cell.The mechanism of anti-invasion may be to inhibit the adhesion,migration,and the CB secreation of the cell.