Studies On The Chemical Constituents Of Pinella Pedatisecta Schott And The Quality Control Of Arisaematis Rhizoma

Arisaematis rhizoma is recorded in Chinese Pharmacopoeia, having function of eliminating stagnation and detumescence. Pinella pedatisecta belongs to genus Pinellia (family Araceae). It is claimed that the rhizome of Pinella pedatisecta can dispel wind, relieve convulsion, dissipate phlegm and eliminate stagnation. Although Pinella rhizoma is not recorded in Chinese Pharmacopoeia, it is commonly used in the market as Arisaematis rhizoma.In the present study, the different chemical constituents between Pinella pedatisecta and Arisaematis rhizoma have been investigated. Five compounds were isolated using various chromatographic methods from the extract of the rhizome of Pinella pedatisecic. Their structures were elucidated on the basis of their MS.'H-NMR, and13C-NMR data. Thev are [(+)-isolariciresinol]. salicylic acid. eudesrmn.p-hydroxy benzaldehyde, and5-methoxysalicvlic acid.Arisaematis rhizoma preparatum has the function of depriving the evil wetness, dissipating phlegm and eliminating wind, can be used for treatment of circumgyration and apoplexy. However, its quality control method is still inadequate to evaluate the overall quality of the herb and its related products. In this study, the quality control method has been established, and the method is validated. Using the established method,13batches of samples purchased from different regions were quantitated.Nowadays, Arisaematis rhizoma have common clinical curative effects are anti-convulsant and anti-tumor. In our study, the nucleotide and linoleic acid types compounds were proved to be the main constituents of Arisaematis rhizoma. Pharmacodynamic experiments indicated that nucleotides could mediate central nervous system and had cytotoxicity. Linoleic acid could soften blood vessel, promote microcirculatory, prevent angiocardiopathy, and also possessed cytotoxicity. Therefore nucleotides and linoleic acid closely related to the clinical curative effects of Arisaematis rhizoma. Using HPLC-DAD method, the experiments of simultaneous determination of adenosine and adenine, Quantitative determination of linoleic acid in Arisaematis rhizoma were carried out. The developed HPLC method was simple, accurate, precise and sensitive, could be readily utilized for the quality control of Arisaematis rhizoma.