The Basic Studies On New Officinal Resources Of The Leaves Of Uncaria Rhynchophylla (Miq.) Jacks.

For the purpose to offer the evidence of exploitation on the leaves of Uncaria spp, investigated the chemical constituents of the leaves of Uncaria rhynchophylla (Miq.) Jacks. , compared the contents of alkaloid in leaves and hooks of Uncaria and studied the preparation technology of the extracts of leaves of Uncaria rhynchophylla (Miq.) Jacks..1. The constituents were isolated by silica gel column chromatography, preparative TLC and Pre-HPLC. Five compounds were isolated from the leaves of Uncaria rhynchophylla (Miq.) Jacks..Their structures were identified as rhynchophylline,isorhynchophylline,corynoxeine,isocorynoxeine and vincoside lactam. The structures were elucidated by physico-chemical characters and spectroscopic analysis such as IR, NMR and MS etc. Isorhynchophylline was isolated from the leaves of Uncaria rhynchophylla (Miq.) Jacks.for the first time. The comprehensive NMR data of isorhynchophylline were given more clearly in this paper.2. The mp. , IR, UV, NMR, MS, TLC chromatograms of 5 standards were mensurated . The results indicated that rhynchophylline,corynoxeine,isocorynoxeine and vincoside lactam were accord with the specification of HPLC. Because of the instability, the purity of isorhynchophylline was only 93.24%.3. A reversed-phase high performance liquid chromatography(RP-HPLC) method wasdeveloped for the assay of 4 oxindole alkaloids (rhynchophylline,isorhynchophylline,corynoxeine and isocorynoxeine) and 1 glycoindole alkaloid (vincoside lactam) in Uncaria spp. The gradient solvent system with 0.05 mol·L^-1 ammonium acetate buffer (pH=5.20) and acetonitrile and ODS reversed-phase column of Kromasil C₁₈(4.6 mm×200mm, 5μm) were used in the quantitative analysis of the 5 marker components. The eluate was monitored at 245nm. The linear ranges of rhynchophylline, isorhynchophylline, corynoxeine, isocorynoxeine and vincoside lactam were 0.84-13.50 mg·mL^-1, 0.86-27.00 mg·mL^-1, 1.58-50.50 mg·mL^-1,1.63-52.00 mg·mL^-1 and 1.47-47.00 mg·mL^-1 (r>0.9995) respectively. The recoveries were 97.3%,101.2%,98.8%,101.4% and 98.8% respectively, and the RSD was less than 2.0%. The assay methods were rapid and with good reproducibility and provided a quantitative basis for the quality compare of the leaves and hooks of Uncaria spp. The results indicated that the kinds and contents of alkaloids were different between the different species; the kinds of alkaloids from different areas were similar to each other, but the contents of alkaloids were different in the leaves and hooks of Uncaria rhynchophylla (Miq.) Jacks..Comparison of the contents of alkaloids with different harvest and processing methods would offer the foundation of the choice of crude drugs. The results indicated that contents of 5 marker components are higher in green leaves hand-picked than in yellow defoliation.4. Studies on the preparation technology of the extracts of the leaves of Uncaria rhynchophylla (Miq.) Jacks.with the yield of extract, the contents of marker components and the availability as the review indexes.