The Hawthorn Leaves Fingerprints Injection Studies

In this paper, 10 samples of the leaves of Crataegus pinnatifida Bge.var.major N.E.Br. were collected from different areas and different batchs. It was identified that all of them were the leaves of Crataegus pinnatifida Bge.var.major N.E.Br. HPLC with DAD detector was used to analyze the flavone in the samples of the leaves of Crataegus pinnatifida Bge.var.major N.E.Br. and rhamnosylvitexin was used as internal standard compound: Chromatography was performed on a Hypersil BDS C₁₈column(250mm×4.6mm, 5μm). Mobile phase consisted of solvent A [acetonitrile-tetrahydrofuran (98:2, V/V)] and solvent B (0.2% orthophosphoric acid) and solvent C ( methanol). It was gradient elution. The UV detector was set at 360nm and the flow rate was 1.0 mL ? min^-1, column temperature 25℃and the injection volume 20μL. Based on this study , HPLC fingerprint analysis method for the leaves of Crataegus pinnatifida Bge.var.major N.E.Br. was developed. Analyzing by Computer Aided Similarity Evaluation system, the similarity of the leaves of Crataegus pinnatifida Bge.var.major N.E.Br. from different areas was more than 0.9.Up to now, the report of hawthorn leaves has focused on the chemical components, pharmacology and clinical study, but there are few studies in the preparation for Powder Injection. So, we studied hawthorn leaves's preparation methods about extraction, isolation and purification for Powder Injection and established methods of the quality control. In investigated process, the amount and content of flavonoids collected were compared, and it's aim that the percentages of the contents of total flavonoids was more than 80%. Hawthorn leaves flavonoids was extracted from hawthorn leaves by using 40% ethanol and isolated by using macroporous resin. By using the content of hawthorn leaves flavonoids as quality control criteria, extraction and isolation process of hawthorn leaves flavonoids in hawthorn leaves was optimized with the aid of orthogonal design and mono-factor trial. It was demonstrated that this method was convenient, economical and suitable for manufacture in industry.Flavonoids in hawthorn leaves in different regions was determined by colorimetry and detected at 500nm. A RP-HPLC method was developed to determinate the content of hyperoside in hawthorn leaves flavonoids .As a result, a good linearity was obtained from 3.44～29.24μg ? mL^-1 , with r=0.9997 for hyperoside. The average recovery was 98.2%, RSD=1.6%(n=9). The method was proved to be simple, precise, sensitive.Based on the study of fingerprints of hawthorn leaves. HPLC method was used to analyze 10 batchs hawthorn leaves flavonoids and hawthom leaves injection respectively. The fingerprints of hawthorn leaves flavonoids and hawthorn leaves injection were set up. Analyzing by computer Aided Similarity of hawthorn leaves flavonoids and hawthorn leaves injection was more than 0.99 respectively. The studies are useful for the quality control of hawthorn leaves flavonoids and hawthom leaves for injection.