| Purpose:Through detecting the content of adenosine acid, the activity of oxidase of mitochondria respiratory chain (COX, NOX)and the anti-oxidation capacity (the activity of SOD, GSH and the content of MDA) in skeletal muscle of rats, we expound the influence rule of the content of adenosine acid,the activity of oxidase of mitochondria respiratory chain, and the oxidation resistance ability in the skeletal muscle during different intensity endurance movement training. From the standpoint of the change of running suitability of the activity of oxidase of mitochondria respiratory chain and the anti-oxidation capacity, we investigate possible mechanism of adaptive changes in energy metabolism of the skeleton muscle; after different intensity endurance trainings.Methods:In this study, refering to the standard of maximal oxygen intake Bedford reported, 40 SD female big mouse were used and divided randomly into four groups:(1)Control-group (C), (2)low-intensity group (L), (3)medium -intensity (M), (4)high-intensity group (H).Each one has 10.Control-group rats were fed normally. Other -group rats set training two day, take a rest for a day, train again for three days and take a rest for a day. It sustains totally 7 cycles. Soleus (SOL) and medial grastrocnemius (MG) of rats in groups and batches were handled after experiment. Determination of ATP, ADP, AMP, in Skeletal Muscle by HPLC; The activities of cytochrome oxidase(CCO), NADH oxidase and the activities of antioxidase SOD, GSH-PX, and MDA were measured by Spectrophotometry.Results:1. Adenosine acid:the levels of ATP,AMP of rat gastrocnemius muscle are higher than that of the soleus muscle. The levels of ADP of rat soleus muscle are higher than that of the gastrocnemius muscle. The levels of ATP,AMP of rat gastrocnemius muscle and the levels of ATP,ADP,EC of rat soleus muscle in LG were significantly higher than that in CG (P<0.05). The levels of EC of rat gastrocnemius muscle in LG were significantly higher than that in CG very much (P<0.01). The levels of ATP,AMP,EC of rat gastrocnemius muscle and the levels of ATP,ADP of rat soleus muscle in MG were significantly higher than that in CG very much (P<0.01). The levels of EC of rat soleus muscle in MG were significantly higher than that in CG (P<0.05). The levels of ATP,EC of rat gastrocnemius muscle in HG were significantly decreased than that in CG (P<0.05～0.01).2. Oxidase of mitochondria respiratory chain:the levels of NOX,COX of rat soleus muscle in LG were significantly higher than that in CG (P<0.05). The levels of NOX,COX of rat gastrocnemius muscle and the levels of NOX of rat soleus muscle in MG were significantly higher than that in CG very much (P<0.05). The levels of COX of rat soleus muscle in MG were significantly higher than that in CG very much (P<0.01). The levels of NOX,COX of rat gastrocnemius muscle in HG were significantly higher than that in CG very much (P<0.01).3. The anti-oxidation capacity:The levels of MDA of rat gastrocnemius muscle and the levels of GSH,SOD of rat soleus muscle in LG were significantly higher than that in CG (P<0.05-0.01). The levels of SOD,GSH,MDA of rat gastrocnemius muscle and the levels of SOD of rat soleus muscle in MG were significantly higher than that in CG (P<0.05). The levels of SOD,GSHof rat gastrocnemius muscle in HG were significantly higher than that in CG very much (P<0.01). The levels of MDA rat soleus muscle in HG were significantly higher than that in CG (P<0.05).4. The contents of EC of rat soleus muscle were very positive significant correlated with COX (P< 0.01) and NOX (P< 0.05). The contents of ATP of rat soleus muscle were very positive significant correlated with COX (P< 0.01).The activity of EC of slow muscle can be regulated by NOX,COX, and the COX activeness is more sensitive,but The activity of ATP of slow muscle can be regulated by COX.5. The activity of NOX of rat soleus muscle was very positive significant correlated with SOD and GSH (P< 0.05). The activity of COX of rat soleus muscle was very positive significant correlated with SOD (P< 0.05) and GSH (P< 0.01). The activity of COX of rat gastrocnemius muscle was very positive significant correlated with GSH (P< 0.05).Conclusions:1. Except to the activity of ATP, EC of skeletal muscle was sensitive to LG and CG training,the activity of ADP was sensitive to slow muscle in LG and CG, but the activity of AMP was sensitive to fast muscle in LG and CG.2. The activity of oxidase of mitochondria respiratory chain and the anti-oxidation capacity in slow muscle of rats in LG were obviously improved. The activity of oxidase of mitochondria respiratory chain and the anti-oxidation capacity in fast muscle of rats in HG were obviously improved.3. After endurance sports training, the activity of SOD and GSH were obviously improved, so that the integrity of cell membrane was assured. The changes of CCO activity is closely related to mitochondrial energy metabolism enhancement, particularly mitochondrial energy metabolism in slow muscle. |
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