| This study in establishing altitude training compound added different doses iron agent based on the model, Through the determination of serum ferritin in the blood of rats (SF), transports iron protein indicators, explore altitude training process the change rule of iron metabolism. Through the determination of liver tissue mitochondrial enzyme complexes respiratory chain NADH-Q oxidoreductase (CI), butanedioic acid-Q oxidoreductase (CII), cell pigment oxidoreductase (CIII) and cell enzyme cytochrome oxidase (CIV) activity, Explore altitude training compound supplement iron agent on the influence of mitochondrial function of respiratory chain, For altitude training and complement iron agent on iron metabolism and the influence of mitochondrial function of the respiratory chain provide reference material.Experimental methods2 months with male health Wister rats for experimental object, After running to Taiwan and plateau screening Will retain 40 mice were divided into 5 groups: The quiet group in plateau environment (C); The exercise group in plateau environment (T); Small dose supplement iron of the exercise group in plateau environment (S); Dosage in supplement iron of the exercise group in plateau environment (M); High-dose supplement iron of the exercise group in plateau environment (L). Mice 3500m plateau in simulated altitude life or repeats load strength training run sets, Irrigation stomach complement FeSO4. 25m/min increasing speed from the 35m/min, Sports time from increasing 30min to 60min. Every training 1, six days a week of training, training 5 weeks. After practice, the rest in often oxygen environment 24h, Run after motion platform will immediately exhaustion after anesthesia rats, take blood and death of bufo liver tissue. Determine each rat weight, exhaustion time, serum ferritin, transferrin, liver tissue mitochondrial respiratory chain CⅠ, CⅡand CⅢ, CⅣactivity.Results of the experiment:1. Mice's weight: With group (C) compared with, T, S, M, L group mice's weight increase significantly (P < 0.01), which fell39.7%, 53.6%, 62.9%, 59.7%. And group (T) compared with: S, M and L group mice's weight growth dropped significantly (P < 0.01), were down 23.1%, 38.6 percent and 33.1% respectively. With group (S) compared with: M group mice's weigh increase was significantly reduced (P < 0.05), decreased 20.1%; L group mice's weigh growth will drop slightly, but no significant differences, fell 13.2%. And With group (M) compared with: L mice's weight, but the growth of increased slightly non-significance difference, heighten 8.7%.2. Mice's exhaustion time: With group (C) compared with: T, S, M, L group mice's ran machine movement to the top of my lungs time was significantly increased (P < 0.01), respectively 656%, increased 331%, 314%, 197%. And the group (T) compared with: Group S, M and L group mice's ran machine movement to exhaustion time shortens significantly (P < 0.01), respectively, 45.2%, shorten 43% colonoscopy With group (S) compared with: L mice's run to the top of my lungs platform movement time shortens significantly (P < 0.01), shortened 31.1%; M mice's run to the top of my lungs platform movement slightly shorter, shorten the time 3.8%. And in the group (M) compared with: L mice's ran machine movement to exhaustion time significantly (P < 0.01), decreased 28.4%.3. Serum iron protein content: With group (C) compared with: T, S, M, L group mice's serum iron protein content have significantly (P < 0.01, P < 0.05), which fell 22.2%, 15.5%, 6.1%, 20.2%. And the group (T) compared with: S and M group mice's serum iron protein content increased significantly (P < 0.01, P < 0.05), respectively, 20.7% 8.7% increased; L group mice's serum iron protein content, increased slightly increase 2.5%. With group (S) compared with: M group mice's serum iron content of protein content of the noticeable increase (P < 0.01), increased 11%; L group mice's serum ferritin content significantly (P < 0.05), fell 5.6%. And in the exercise group supplement iron doses (M) compared with: L group mice's serum ferritin content significantly (P < 0.01), dropped 15%.4. Serum transferring content: Compared with the group C, the mice's serum transferring content of S, M, L group have significantly (P < 0.01), were down 5.6%, 6.6%, 8.9%, The transferrin content of T group have slightly low, but no significant difference. Compared with the group T, the mice's serum transferrin content of S, M, L group have significantly (P < 0.01), which fell 5.1 %, 6.0%, 8.3%. Compared with the group S: mice's serum transferring content of M group is no significant difference, slightly lower 0.98%; mice's serum transferring content of L group have significantly (P < 0.05), dropped 3.4%. Compared with the group M: the serum transferrin content of L group have significantly (P < 0.05), fell 2.4%.5. Respiratory chain enzyme complex activity: Compared with the group C, the activities of CⅠ, CⅡof T group were improved significantly (P < 0.01), up respectively 130.4%, 81%; the activities of CⅢ, CⅣwere dropped significantly (P < 0.01, P < 0.05), were down 22.5%, 22.3%. The activities of CⅠ, CⅡ, CⅢ, CⅣof S Group were improved significantly (P < 0.01, P < 0.05), respectively increase 134.8%, 55% 16.2%, 26.5%. The activities of CⅠ, CⅢ, CⅣof M group were improved significantly (P < 0.01, P < 0.05), and respectively growth 80%, 26.8%, 18.9%; the activity of CⅡdeclined slightly, fell 14.5%, no significant differences. The activities of CⅠ, CⅣof L group were improved significantly (P < 0.01) growth 67.6%, 18.3%; the activity of CⅡwas increases a little growth 4.3%, no significant differences; the activity of CⅢwas significantly (P < 0.01), decreased 37.5%.Compared with T group, the activities of CⅢ, CⅣof S group were improved significantly (P < 0.01), respectively growth; 100%, 58.6%, the activity of CⅡincreases a little growth 1.9%, but no significant differences; the activity of CⅠactivity dropped significantly (P < 0.05), descend 35.8%. The activities of CⅢ, CⅣof M group were improved significantly (P < 0.01), respectively increase 133.3%, 59%; the activities of CⅠ, CⅡwere reduced significantly (P < 0.01), reduced respectively 34.3%, 62.8 %. The activity of CⅣimproved significantly (P < 0.01), growth out; the activity of CⅡsignificantly reduced (P < 0.01), reduced 54.7%; the activities of CⅠ, CⅢwere reduced, reduced respectively 7.5%, 19.4%, but no significant differences.Compared with the group S, the activities of CⅠ, CⅢ, CⅣof M group, growth slightly improve 2.3%, 0.26% 4.0%, but no significant differences; the activity of CⅡhave significantly lower (P < 0.01), reduced 63.6%. The activity of CⅠof L group improved significantly (P < 0.05), growth 44.2%; the activity of CⅡ, CⅢreduced significantly (P < 0.01), reduced 55.6%, 59.7%; the activity of CⅣreduce 6.5 %, but no significant differences.Group compared with M, the activity of CⅠof L group improved significantly (P < 0.01), growth 40.9%; the activity of CⅡ, rise slightly improve 22%, but no significant differences; the activity of CⅢhave significantly lower (P < 0.01), reduces 65.3%; the activity of CⅣdecreases, reduced 6.7%, but no significant differences.Conclusion:(1)Pure altitude training to significantly inhibit the growth of rat's weight, Altitude training compound complement iron agent can be more significant inhibition of rat's weight growth, And in the exercise group supplement iron dose the most obvious effect.(2)Pure altitude training can significantly extend rat exhaustion, athletic time The longest in plateau, altitude training speed-training compound complement iron agents can also significantly extend in all sports time, top supplement iron agent in small doses of exercise group supplement iron the longest, soil. In doses(3)Each group rat's serum iron group compared with quiet protein content of significantly down. With simple plateau group serum iron protein content of the lowest, filling iron in speed-training, take iron supplements group dose serum iron protein content of the most close to quiet, it can be seen that the exercise group supplement iron in the dosage is highest, small doses of iron reserves soil.(4)Altitude training compound in take iron supplements can effectively regulate the dosage, metabolic disorder iron.Small doses of component. Pure plateau in regulating iron speed-training, metabolic disorder. No obvious function.(5)Each group are different degree of improved exhaustion after exercise rat hepatic mitochondria immediately enzyme activity, including respiratory chain, with altitude training compound in best supplement iron, dosage small doses component.In conclusion, in improving the iron metabolism, maintain iron reserves aspects: altitude training compound in doses supplement iron group is the best, small doses of filling iron poorer; Pure plateau in the regulation of iron metabolism dyslalia evaluation and iron reserves far lower than other groups in. In improving exhaustion after exercise rat hepatic mitochondria immediately respiratory chain enzyme activity aspects: altitude training compound in best supplement iron, dosage small doses component. |
PDF Full Text Request