| Experimental methodIn February healthy male Wister rats aged as experimental object, screening out therats were divided into normal oxygen control group (C), HiHiLo1week traininggroup (H1), HiHiLo2week training group (H2), HiHiLo3week training group (H3),HiHiLo4week training group (H4), HiHiLo5week training group (H5). By usingself-made hypobaric oxygen chamber caused by hypobaric hypoxia (oxygen partialpressure of15.4%, equivalent to an altitude of2500m height), normoxic trainingabove sea level,1500m (with the city of Lanzhou is the altitude base point), trainingday the next day add a hypoxic condition training; rat12h daily life in a low-oxygenenvironment,12h living in normoxic environment (includes a training day). Normoxiccontrol rats fed a week after sampling, training group by grouping all time in week1material, were used to detect the rat body weight, serum creatine kinase (CK)activity, skeletal muscle mitochondrial ATP enzyme activity, skeletal musclemitochondrial respiratory chain enzyme complexes of I, II, III, IV activity indexes.The experimental results1rats serum creatine kinase (CK) activity in second weeks to reach a maximumvalue, of which the first three weeks compared with group C significantly increased(P <0.01, P <0.05), were increased by35.23%(H1group),61.93%(H2group),32.39%(H3group); group H2CK activity reaches a value the highest,4;H3,5group was significantly decreased, and H2group decreased significantly, reducedby18.25%(H3group),32.98%(H4group),34.74%(H5group).2H1rats skeletal muscle mitochondrial respiratory chain enzyme complexes of I, II,III, IV activity compared with the C group were significantly decreased, respectively30.03%,35.56%,14.54%,35.08%.(P <0.01, P <0.05); H2group except enzymecomplexes of III had no significant difference (down2.10%) outside, the other threeactivity of enzymatic complexes were significantly lower than in control group (P <0.01, P <0.05), drop respectively16.49%,13.73%,24.97%; H3rats skeletal musclemitochondrial respiratory chain complexes in I than in C group increasedsignificantly15.63%. While the enzyme complexes of II, III, IV in C group comparedwith H4group had no statistical significance; skeletal muscle mitochondrial enzyme complexes of I, II, IV activity significantly increased (P <0.05),respectively17.36%,17.61%,37.13%,21.44%; H5group skeletal muscle mitochondrialenzyme complex activity of I, II, III, IV activity was significantly increased (P <0.01), respectively42.10%,39.08%,49.74%,35.83%.3in skeletal muscle mitochondrial ATP enzyme activity showed decreased first andthen increased to tend stable. Compared with group C, group H1mitochondrial ATPenzyme activity decreased significantly (P <0.01), decreased39.27%; H2groupdecreased21.30%(P <0.05); H3group and the control group compared with H4group increased8.91%; skeletal muscle mitochondrial ATP enzyme activityincreased40.21%(P <0.01); group H5of skeletal muscle mitochondria the activity ofATP increased by38.39%(P <0.01).Conclusion1first weeks,2weeks of elevated creatine kinase activity, the beginning of the thirdweek of decline, fourth weeks,5weeks continued to decline, in the process of HiHiLoskeletal muscle adaptation from the beginning of the third week.Week2.5HiHiLo could significantly improve the skeletal muscle mitochondrialrespiratory chain C I~IV activity, in the process of HiHiLo, skeletal musclemitochondrial respiratory chain enzyme complex enzyme I~IV activity weredecreased in the first week, starting from second weeks to fifth weeks continued torise, the skeletal muscle mitochondrial respiratory chain C I, C III in third weeks havebeen higher than quiet when the activity of the enzyme, that skeletal musclemitochondrial respiratory chain enzymes required to3-4Zhou Ke HiHiLo.3.HiHiLo can not only improve the quiet when skeletal muscle mitochondrial ATPenzyme activity, but also can enhance the activity of the reserve force, so as toenhance skeletal muscle contraction. Skeletal muscle mitochondrial ATP enzymeactivity at third weeks than in quiet, that skeletal muscle mitochondrial ATP enzymeswithin3weeks had finished HiHiLo acclimatization... |
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