A Research On The Impact And Mechanism Of Different Hypoxic Training On Enzymatic Activity Of Skeletal Muscle's Respiratory Chain Of Rats

Altitude training on the body is a complex physiological effect and the training effect.However,disadvantages as well as altitude training,in order to overcome the negative effects of altitude training and shortcomings,and scholars in the altitude training on the basis of a large amount of simulated hypoxic training theory and practice.Is one of the most prized is HiHiLo hypoxic training mode,the training way either through hypoxic exposure to improve the athletes' oxygen transport and the ability to use oxygen,and can sustain the movement of the normal strength training,but also through the low oxygen environment movement improve athletes' cardiopulmonary function,therefore,is a more comprehensive to provide the methods of physical fitness.This experiment mainly from three aspects:(1)the comparative research on constant load low living practice,high living practice training on skeletal muscle of rats respiratory chain enzyme activity and the influence of mechanism;(2)comparative study on increasing load low living practice,high living practice training on skeletal muscle of rats respiratory chain enzyme activity and the influence of mechanism;(3)comparative study under the same hypoxic training method,a constant load,increasing load of skeletal muscle of rats respiratory chain enzyme activity and the influence of mechanism;Study different load hypoxic training on skeletal muscle of rats respiratory chain enzymes and their genetic and other related,the influence of training for the low oxygen to provide better and more scientific training plan.Experimental MethodsSelect 50 weight about 150 g of the healthy male Wistar rats as experimental object,will be randomly divided into 6 groups,8 rats in each group,a total of five weeks training.Oxygen often quiet quiet(C)and hypoxia group(D)no training;Low living practice training group(S-LoLo)constant load rate of 39 m/min,30 min,a total of 6 times,slope is 0,living in constant oxygen environment;Low living practice of increasing load training(P-LoLo)incremental training,at the rate of 35 m/min 1week,2 weeks to 36 m/min speed,rate of 37 m/min 3 weeks and 4,and 5 weeks for39 m/min speed,movement 30 min,a total of 6 times,slope is 0,living in constant oxygen environment;High living high training low xun constant load training group(S)-HiHiLo often oxygen constant oxygen training speed of 39 m/min,a total of6 times,the next day with low oxygen constant training speed is 34 m/min,a total of 3times,slope is zero,remove the training time,rats were living under normal oxygen and low oxygen environment 12 h;High living high training low training increasing load training group(P-HiHiLo)often oxygen increasing at the rate of the first week of training week 1 35 m/min,the rate of 36 m/min 2 weeks and 3 weeks at the rate of37 m/min,at the rate of 39 m/min.4,5 weeks,a total of 6 times,the next day with low oxygen increasing speed of the first week of training for the 30 m/min,the rate of31 m/min 2 weeks and 3 weeks to 32 m/min speed,4,5 weeks speed is 34 m/min,a total of 3 times,30 min,slope is zero,remove the training time,rats were living under normal oxygen and low oxygen environment for 12 h.Often oxygen in lanzhou city of altitude,low oxygen is 3500 m above sea level.Five weeks after the training,beheaded kill,and quadriceps and take blood serum creatine kinase(CK),mitochondrial complex enzyme ?-?,SOD,MDA,complex enzyme ? COX? index.The result(1)in rats after five weeks of hypoxic training of different load,different amplitude of weight gain,rather high living high training low training increasing load training group P-HiHiL oweight decreased in a certain extent.(2)after five weeks of hypoxic training of different load,the rat serum creatine kinase activity produced change.Compared with the C,D,there was no significant difference(P > 0.05);S-LoLo,P-HiHiLo LoLo,S and P-HiHiLo is extremely significant difference(P < 0.01),S-Lo Lo group rose 30.93%,the P-Lo Lo group rose 23.40%,S-HiHiLo rose 51.27%,the P-HiHiLo group rose 39.53%.Compared with D,S-LoLo,P-LoLo,S-HiHiLo,P-Hi Hi Lo extremely significant differences(P < 0.01),S-Lo Lo group rose 28.69%,the P-LoLo group rose 21.29%,S-HiHiLo rose 48.68%,the P-HiHiLo group rose 37.14%.Compared with S-LoLo,P-LoLo there were significant differences(P < 0.05),S-HiHiLo and P-HiHiLo is extremely significant difference(P < 0.01),P-LoLo group fell 5.76%,S-HiHiLo rose 15.53%,the P-HiHiLo group rose 6.56%.Compared with P-Lo Lo,S-Hi Hi Lo and P-HiHiLo is extremely significant difference(P < 0.01)S-Hi Hi Lo rose 22.59%,the P-HiHiLo group rose13.07%.Compared with S-HiHiLo,P-HiHiLo is extremely significant difference(P <0.01),down 7.76%.(3)in rats after five weeks of different hypoxic training load,the rat skeletal muscle mitochondrial respiratory chain complex enzymes ?-? activity are changed.Compared with the C,S-LoLo rat skeletal muscle mitochondrial respiratory chain complex enzymes ?-? activity were increased by 28.13%,7.93%,6.14%,37.92 %(P < 0.01,P < 0.05),P-Lo Lo rat skeletal muscle mitochondrial respiratory chain complex enzymes ?-? activity were increased by 33.59%,27.11%,28.14%,40.20%(P < 0.01,P < 0.05),S-HiHiLo rat skeletal muscle mitochondrial respiratory chain complex enzymes ?-? activity were increased by 39.06%,49.87%,3.42%and 76.04%(P < 0.01,P < 0.05),P-HiHiLo rat skeletal muscle mitochondrial respiratory chain complex enzymes ?-? activity were increased by 68.36%,78.26%,16.86% and 94.79%(P < 0.01,P < 0.05).Compared with D,S-Lo Lo rat skeletal muscle mitochondrial respiratory chain complex enzymes ?,?,? activity were increased by 19.27%,31.74%,12.66%,and compared with compound ? no statistical significance(P < 0.01,P < 0.05,P >0.05),P-LoLo rat skeletal muscle mitochondrial respiratory chain complex enzymes?-? activity were increased by 24.36%,19.76%,59.04%,13.87%(P < 0.01,P <0.05),S-HiHiLo rat skeletal muscle mitochondrial respiratory chain complex enzymes ?-? activity were increased by 29.45%,42.93%,28.37%,42.98%(P <0.01,P < 0.05),P-HiHiLo rat skeletal muscle mitochondrial respiratory chain complex enzymes ?-? activity were increased by 56.73%,70%,45.04%,58.21%(P < 0.01,P < 0.05).Compared with S-LoLo,P-LoLo rat skeletal muscle mitochondrial respiratory chain complex enzymes ?-? activity were increased by 4.27%,17.77%,20.73%and 1.66%(P < 0.01,P < 0.05),S-HiHiLo rat skeletal muscle mitochondrial respiratory chain complex enzymes ?,?,? activity were significantly increased by 8.54%,38.86%,27.64%,and the compound ? fell by 2.56%(P < 0.01,P < 0.05),P-HiHiLo rat skeletal muscle mitochondrial respiratory chain complex enzymes ?-? activity were increased by 31.40%,65.17%,10.09%,41.24%(P < 0.01,P <0.05).Compared with P-LoLo,S-HiHiLo rat skeletal muscle mitochondrial respiratory chain complex enzymes ?,?,? activity were increased by 4.09%,17.90%,25.56%,and the compound ? fell by 19.28%(P < 0.01,P < 0.05),P-HiHiLo rat skeletal muscle mitochondrial respiratory chain complex enzymes ?,?,? activity were increased by 26.02%,40.24%,38.93%,and the complex ? fell by8.81%,(P < 0.01,P < 0.05);Compared with S-HiHiLo,P-HiHiLo rat skeletal muscle mitochondrial respiratory chain complex enzymes ?-? activity were increased by 21.07%,18.94%,12.98%,10.65%(P < 0.01,P < 0.05).(4)in rats after five weeks of different hypoxic training load,the rat skeletal muscle mitochondria SOD activity and MDA content changes.Compared with the C,S-LoLo skeletal muscle mitochondria 14.97% increase SOD activity,MDA content decreased by 9.49%(P < 0.01),P-LoLo skeletal muscle mitochondria 17.50%increase SOD activity,MDA content decreased by 15.42%(P < 0.01),D skeletal muscle mitochondria 1.93% increase SOD activity,MDA content decreased by 1.19%,but not statistically significant(P > 0.05),S-HiHiLo skeletal muscle mitochondria SOD activity increased by 32.42%,decrease MDA content 20.51(P < 0.01),P-HiHiLo skeletal muscle mitochondria SOD activity increased by 42.36%,decrease MDA content by 30.60%(P < 0.01).Compared with D,S-LoLo skeletal muscle mitochondria 12.79% increase SOD activity,MDA content decreased by 8.49%(P < 0.01),P-15.28% Lo Lo skeletal muscle mitochondria increased SOD activity and MDA content decreased by 14.01%(P < 0.01),S-HiHiLo skeletal muscle mitochondria 29.92% increase SOD activity,MDA content decreased 19.56%(P < 0.01),P-HiHiLo skeletal muscle mitochondria38.18% increase SOD activity,MDA content decreased 29.76%(P < 0.01).Compared with S-LoLo,P-LoLo skeletal muscle mitochondria 2.20% increase SOD activity,MDA content decreased by 6.55%(P < 0.05),S-HiHiLo skeletal muscle mitochondria 15.18% increased SOD activity and MDA content decreased12.18%(P < 0.01),P-HiHiLo skeletal muscle mitochondria 22.51% increase SOD activity,MDA content decreased 23.32%(P < 0.01).Compared with P-LoLo,S-HiHiLo skeletal muscle mitochondria SOD activity increased by 12.69%(P < 0.01),MDA content decreased by 6.60%(P < 0.05),P-HiHiLo skeletal muscle mitochondria 19.86% increase SOD activity,MDA content decreased 17.94%(P < 0.01).Compared with S-Hi Hi Lo,P-HiHiLo)skeletal muscle mitochondria 6.36%increase SOD activity,MDA content decreased 12.69%(P < 0.01).(5)in rats after five weeks of different hypoxic training load,complex enzyme? subunits COX ? amount of gene expression changes.Compared with the C,S-Lo Lo,P-LoLo,D,S-HiHiLo and P-HiHiLo is extremely significant difference(P< 0.01),S-LoLo group rose 70.50%,the P-LoLo group rose 137.20%,71.10% rise in group D,S-HiHiLo rose 188.20%,the P-Hi Hi Lo group rose 244.60%.Compared with D,S-LoLo there was no significant difference((P > 0.05)),fell by 0.35%,P-LoLo,S-HiHiLo,P-Hi Hi Lo is extremely significant difference(P <0.01),P-LoLo group rose 38.63%,S-HiHiLo rose 64.93%,the P-HiHiLo group rose 101.40%.Compared with S-Lo Lo,P--HiHiLo Lo Lo,S and P-HiHiLo is extremely significant difference(P < 0.01),P-Lo Lo group rose 39.12%,S-HiHiLo rose65.51%,P-HiHiLo set up 102.11%.Compared with P-Lo Lo,S-HiHiL and P-Hi Hi Lo extremely significant differences(P < 0.01),S-HiHiLo group rose 18.87%,the P-HiHiLo up 45.28%).Compared with S-HiHiLo,P-HiHiLo is extremely significant difference(P <0.01),P-HiHiLo group rose 22.11%.In Conclusion1.The increasing load training in hypoxic training training method to maintain the balance of body weight of rats with good results.2.Under the different load hypoxic training,P-HiHiLo serum creatine kinase activity change is most obvious,that P-HiHiLo training ability to resist fatigue,enhance the recovery of the body,make the body get used to it.3.Different load hypoxic training can improve the rat skeletal muscle mitochondrial respiratory chain complex enzymes activity,strengthen mitochondrial respiratory function and energy metabolism level was improved.P-HiHiLo is most obvious in movement patterns.4.The body of antioxidant enzyme activity and the content of free radical complement each other.The same low oxygen different training methods,training load incremental load with a constant load effect is good;The same training load of different hypoxic training method,high living practice low training Hi HiLo is more advantageous to eliminate free radicals,maintain the balance of the good antioxidant enzymes and free radicals.5.Different load hypoxic training can promote the complex enzyme ? subunits COX ? gene expression,and improve the activity of respiratory chain enzymes,promote the improvement of sports.