Limit Load Movement Of The Rat Frontal Ncam Mrna Expression And Spatial Memory

Objective Water maze training in rats 10 days after its load of an extreme sports processing, observation of Neuronal adhesion molecule (NCAM) mRNA expression and changes in water maze latency, discussion the movement of a limit load effect on the expression of NCAM mRNA in rat frontal cortex and memory maintenance and Retrieval, to provide a scientific theory reference For sports training, fitness and sports rehabilitation. Methods Morris water maze temperature behavior of the SD rats were trained, and then swimming to weight-bearing weight of a 5% load of extreme sports were treated with a limit load movement dynamic effects on memory and using real-time PCR research the dynamic expression of NCAM mRNA in rat frontal. Results 1.Water maze training exercise after the limit load expression of NCAM mRNA treated with the control group (C) compared with immediate group (E1) 0.67 times higher than the control group, 12-hour group (E2) 0.68 times higher than the control group, 24-hour group (E3) 0.36 times higher than the control group, 48 hours group (E4) 2.41 times higher than the control group, E4 and C, E1, E3 comparison, P <0.05; only behavior for water maze training group NCAM mRNA expression compared with the control group ( C), instant group (W1) 0.56 times higher than the control group, 12 h (W2) 0.95 times higher than the control group, 24h group (W3) 1.04 times higher than the control group, 48 hours group (W4) 1.36 times higher than the control group, statistical analysis, P greater than 0.05, but, W1, W2 and E4 comparison P <0.05; order to observe the final behavior will affect the detection of NCAM mRNA expression, we observe the water maze trained rats after a limit load movement, The behavioral test is not directly derived (E) and extreme sports in the water maze test after the load immediate group (E1) of the expression, the former is 0.87 times the control group, which is 0.67 times the control group, P> 0.05. 2. Water maze latency time: After 10 days of water maze training, each group of space exploration significantly reduced latency time, each group on day 10 compared with 1 day incubation period, P values less than 0.01. After the limit load of treatment, in the corresponding time points, in addition to the load limit of 12 hours after incubation longer than 12 hours after water maze training short, at 24 and 48 hours of incubation were higher than the limit load of treated water maze training group , immediately after the limit load group (E1) load limit of the incubation period longer than 12 hours after treatment (E2), 24 hours (E3), 48 hours (E4) and immediately after water maze training (W1), 12 hours (W2) , 24 hours (W3), 48 hours (W4) was significantly longer, P values were less than 0.01, and, after water maze training exercise extreme load handling and simple water maze training group latency and incubation period of 10 days compared positioning navigation, ultimate load immediately after group P <0.01, other groups P> 0.05. 3. platform across the region number: extreme exercise stress test and after treatment were compared after 10 days water maze training, extreme sports groups across the load times immediately after treatment was significantly reduction, P <0.01, comparison between two other groups, P> 0.05; water maze test immediately after training, 12,24,48 hours a pair wise comparison P> 0.05, compared with 10 days in water maze training, the two tests there was no significant difference; water maze training limit load motion processing instant group (E1) and 12 (E2), 24 (E3), 48 (E4) hours and water maze training test immediately (W1), 12 ( W2), 24 (W3), 48 (W4) hours of comparison, P <0.01, among the other groups there was no significant difference. Conclusions 1. A limit load exercise training can affect the rat frontal NCAM mRNA expression of rats after the water maze training, NCAM mRNA expression decreased at first and then increased trend in the expression, it was the lowest in 24 hours and the highest expression in 48 hours, while the water maze training NCAM mRNA expression in decreased in the immediate and then 12 hours began to increase, the highest expression of 48 hours, it is said NCAM mRNA expression of treatment with a load of extreme sports can be delayed recovery occurred. 2. one time limit exercise damage to rat water maze training dealing with the formation of spatial memory extraction representation, spatial cognitive ability reduced, this effect immediately after exercise in the performance of limit load of the most obvious can be restored after 12 hours.