| Twenty four 12-month-old, 400-450g weight male Sprague-Dawley rats were obtained from animal institute of Chinese academy of medical sciences. Food and water were available ad libitum. Eight months later, the animals were randomly assigned to an exercise or sedentary group. The trained rats underwent a 12-week endurance swimming training (1 h/day, 5 days/week for the first 5 weeks; 1.5 h/day, 6 days/week for the last 7 weeks) in water at 30-35 ℃. To test the effect of a single bout of exercise (which does not alter body fat mass) on leptin expression, at the end of 12th week, both the two groups were randomly divided as control or acute exercise group. Acute exercise was performed on a rodent treadmill up to 64% peak oxygen uptake, lasted 30 min. Before being killed, the rats were measured body weight and body length. Epididymal, preperetoneal, retroperitoneal and interscapular depots were excised. The wet weight of these adipose depots was determined. Body fat content was measured by regression equation. RT-PCR was used to test the ob gene mRNA level in the four adipose tissue depots. After endurance training, exercised rats had significantly lower body weight, body fat percent , ob gene mRNA levels in epididymal, and interscapular tissues compared to sedentary rats, however the Lee index is not significant different. Sedentary control animals had lower (P? |
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