| Bioactive substances are widely used in food and pharmaceutical industries, because of their excellent biological activity. The bioactive substances mainly existed in complex mixture systems. So it is particularly important to adopt appropriate methods for the extraction, separation, and purification of the bioactive substances. Liquid membrane (LM) separation technology is studied on extraction and separation from20century60decades. This technology, characterised by fast mass transport, high separation efficiency, great selectivity, convenient operation, and easy automatization, has exhibited excellent characters on many fields, such as biological engineering, hydrometallurgy, chemical engineering, extraction, and separation metal ions and organic pollutant from the industrial wastewaters. However, there are few papers on study of the extraction and separation of bioactive substance with liquid membrane technology. This paper briefly introduced the liquid membrane technology and its mechanism and applications on separation of the bioactive substances.The detail works are summarized as follows:1. The paper introduced several methods for the separation and extraction of bioactive substance, especially liquid membrane technology.2. A method on the extraction of amino acids (Leu, Phe, Trp, and Met) through the strip dispersion hybrid liquid membrane (SDHLM) coupled high performance liquid chromatography analysis is developed. Kerosene, di-(2-ethylhexyl) phosphoric acid (D2EHPA), and hydrochloric acid are used as the membrane solvent, the carrier, and the stripping phase, respectively. The fundamental parameters influencing the transport of amino acids, pH in the feed solution, the concentration of the carrier, the volume ratio of stripping solution/organic solution, the type and the concentration of the stripping aqueous phase, and both the flow rate of feed phase and the stripping phase, were explored.3. Gold nanoparticles carrier mediated (AuNPs-CM)-BLM was developed for the separation and extraction of theobromine, theophylline, and caffeine. The optimized conditions were as:the organic phase was2.22g/L AuNPs-TOAB-toluene, the extraction time and stripping time both were20min, pH in the feed solution was11.5, the stripping solution was0.4mol/L HCl, the stirring speed was500rpm, and the temperature was308K. Under the optimal conditions, high separation efficiency was reached. The results indicate that AuNPs-CM-BLM can be successfully applied to the separation and extraction of theobromine, theophylline, and caffeine.4. Silver nanoparticles carrier mediated (AgNPs-CM)-BLM was developed for the sepatation and extraction of theobromine, theophylline and caffeine. The synthesis conditions of silver nanoparticles, the kind of the carrier in organic solvent, the extraction time, the concentration of carrier in the organic phase, pH in the donor phase, the composition of the acceptor solution, the stirring rate, and the temperature were optimized. The organic phase was1.21g/L AgNPs-TOAB-toluene, the extraction time was20min, the stripping time was30min, pH in the feed solution was11.5, the stripping solution was0.6mol/L HCl, the stirring speed was500rpm, and the temperature was308K. Under the optimized conditions, high separation and extraction efficiency were reached. It proved this method to be an efficient extraction method, which can be applied to the separation and extraction of theobromine, theophylline, and caffeine. |
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