Analysis Of Ingredients And Study Of Essential Oil In Different Kinds Of Flos Chrysanthemi

The contents of water, ash, crude fiber, crude fat, crude protein, total flavonoids,Chlorogenic Acid, total sugars, free sugars, free amino acid in11kinds of chrysanthemumwere analyzed in order to have an entire research on chrysanthemum. Meanwhile theGC-MS fingerprints of Gongju was established, the components of volatile oil in Gongjuand other kinds of chrysanthemum were analyzed and compared, which provides scientificbasis for utilization and research on chrysanthemum.The contents of basic nutritions were detected relied on GB-5009.2003.The resultsshowed that the content of water in chrysanthemum were in the range of9.20～17.70g/100g, Hang baiju showed the highest content,while Yeju showed the lowestcontent. The average content of water was12.34g/100g. the content of ash inchrysanthemum were in the range of5.40～9.30g/100g, Qi yueju showed the highestcontent, while Gongju showed the lowest content. The average content of ash was7.12g/100g. the content of crude fat in chrysanthemum were in the range of4.20～12.86g/100g, Huangju showed the highest content,while Gongju showed the lowestcontent. The average content of crude fat was8.20g/100g. the content of crude protein inchrysanthemum were in the range of12.73～20.19g/100g, Chuju showed the highestcontent,while Boju showed the lowest content. The average content of crude protein was14.73g/100g. the content of crude fiber in chrysanthemum were in the range of10.45～20.23g/100g, Qi yueju showed the highest content,while Hang baiju showed the lowestcontent. The average content of crude fiber was13.94g/100gThe important functional components in chrysanthemum were detected. The results isthat the content of total flavonoids in chrysanthemum were in the range of1.09～5.23g/100g Qiju showed the highest content, while Yeju showed the lowest content.Followed by the rest: Boju, Qi yueju, Jin xiangju, Chuju, Huangju, Huaiju, Gongju. Thecontent of chlorogenic acid in chrysanthemum has little difference. Jin xiangju and Yejushowed the highest content. The content of chlorogenic acid in other chrysanthemum wasabout0.4g/100g. The content of total sugars in chrysanthemum were in the range of13.86～34.09g/100g, Gongju showed the highest content, while Qi yueju showed thelowest.The experiments were conducted with HPLC methods to determine the contents offree sugars and amino acids in various species of medicinal Flos chrysanthemi. The resultsshowed that the main free sugars in Flos chrysanthemi were fructose and glucose; there were no sucrose and maltose. The total contents of free sugars in Flos chrysanthemi werein the range of9.01～18.76g/100g. The highest content of total free sugars was found inGongju, while the lowest content in Huangju The total levels of free amino acids in Floschrysanthemi range from2.806to0.563g/100g, among which Qi yueju showed thehighest level of total free amino acids, Hang baiju showed the lowest level of total freeamino acids.The fingerprint analysis method was established to research on the volatileconstituents of Gong Chrysanthemum morifolium in Huangshan City by using GC-MS andComputer Aided Similarity Evaluation. The results showed that the optimum condition ofGC-MS was applied with a fiber column （0.25mm*30m*0.25μm） coated withHP-5MS.The temperature at feed inlet was260℃, the carrier gas was nitrogen, the flowrate was1.0mL·min^-1, the split ratio was25:1, and the sample size was2μL. Theessential oil in ten batches of Gongju was analyzed by adopting temperature programmer.In this condition,59common peaks were identified and GC-MS Fingerprint of59commonpeaks was established preliminarily. The correlation coefficients of ten batches of Gongjuwere higher than0.9. The content of Bicyclo[3.1.1]hept-2-en-4-ol-2,6,6-trimethyl-,aceatein volatile oil of Gongju was highest （20%）. The method is of high degree of precision,reproducibility, and stability. The separating degree of each component in the essential oilis good. Both the established fingerprints and the component ofBicyclo[3.1.1]hept-2-en-4-ol-2,6,6-trimethyl-,aceate could be used as the quality controlindexes for Gong Chrysanthemum morifolium in Huangshan City.From Jin zhuling Gongju, Shangfeng Gongju, Shi menhe Gongju, Jin gongju,Qi yueju and Jin xiangju199、206、201、199、223and237chromatographic peakswere detected, among them49、94、106、96、121and110components were identified, which were composed about89%、66%、82%、75%、82%and77%of totalessential oil respectively. The main kinds of components of volatile oil in chrysanthemum were similar. Monterpenes, sesquiterpenes and their oxo-derivatives were themajor chemicals in the essential oils.