Study On Tissue Culture And Flowering In Vitro Of Gazania Rigens L.

Gazania rigens L. which is also known as medal flower is a variety of perennialherbaceous plants of genus Gazania, Compositae. The colorful corolla as well as thebright petals that open in the morning and close at night makes Gazania rigens L. aperfect candidate for park or garden plants or flower arranging. The seed propagation,plant division propagation and cutting propagation can not meet the consumerdemands. Research on tissue culture and rapid propagation of Gazania rigens L. isimportant since rare relative result has been reported at present. Vitro flower has highornamental and scientific research value which is also another application of tissueculture technology. Plant flowering in vitro is more and more popular on the marketrecently. Gazania rigens L. is one kind of perfect vitro plants for research andpromotion. At present, there is no report about Gazania rigens L. flowering in vitro.The research of Gazania rigens L. flowering in vitro is important in development ofboth theory and application.In this experiment, leaves of Gazania rigens L. were used as explants to induceadventitious buds and rooting. This paper discussed the method of hardening-seedlingand transplanting and built up systems of tissue culture and rapid propagation. Usedadventitious buds of Gazania rigens L. as the test material through different mediumof flowering induction, Exploration flowering in vitro conditions, the systems offlowering in vitro was built up, as well as making a simple analysis of the flower budsdifferentiation regulation. The main results were as follows:1. The best sterilization pattern: sterilized by10%Naclo for15min, and thesuccess rate was88.6%.2. The best medium on the induction of adventitious buds: MS+0.8mg·L^-1TDZ+0.1mg·L^-1NAA. The induction rate reached94.7%.3. The best rooting medium:1/2MS+0.2mg·L^-1NAA+0.5mg·L^-1IBA, therooting rate reached100%. 4. The best soilless media: nutritious soil: vermiculite （1:1）, the living rate couldreach96.7%, meanwhile, seedlings grew strongly, and leaves glittered and all of theiraxes grew upstanding.5. When6-BA and NAA were combined in level of0.5mg·L^-1-0.10mg·L^-1, theinduction rate of flowering was48.9%in60d that reaching the highest point. Adding6-BA alone would inhibit flower formation, and even affected flower morphology.6. Increasing the sucrose concentration moderately had promoting influence inthe induction rate of flowering which reached highest point51.1%when the sucroseconcentration was40g·L^-1. The Flowers opened normally.7. A certain concentration activated carbon （AC） had promoting influence in theinduction rate of flowering. AC inducer took Gazania rigens L. slender stem, smallerbuds and topping on the bottle sealing membrane and hindered the opening of theflower as a result.8. PP333and CCC are growth inhibitor that promoted the induction rate offlowering with the appropriate concentration. The induction rate of flowering was upto54.4%when PP333was0.4mg·L^-1.As a contrast the induction rate was highest50.2%when CCC was0.7mg·L^-1.9. The treatment group had earlier and higher peak value as well as higher buddifferentiation rate than control group which demonstrated that soluble sugar andsoluble protein were the material base of the flower bud differentiation, and PODcontent showed a positive correlation with it.