| In order to seek botanical pesticides for treating Carposina sasakii Matsmura, a research was done to sieve out something edible, that would make the disinsection , oviposition deterrence and ovicidal action effects on Carposina sasakii Matsmura adults, from vegetables and spices(Such as Chinese prickly ash, fennel, coriander, celery,ginger,aniseed and cinnamon). The activities of the effects of the seven plants above on Carposina sasakii Matsmura were tested .Select the best one considering a variety of factors such as insecticidal effect, ecology and economy , and then analyse its composition. All these offer a theory basis for the Carposina sasakii Matsmura control and the exploitation of highly effective pesticide in food. The results showed that:1.Among the seven non-host plants, the inhibiting rates of Chinese prickly ash, celery, cinnamon and aniseed to PFB fecundity were more than 50%, in which the rate of aniseed was up to 100% and the rate of cinnamon was 91.55%. Some volatiles also influenced the PFB fertility rate. The volatile from star anise and cinnamon was the best which could made the PFB fertility rate to reduce to 0%and 13%, which are markedly different with control and other non-host plant volatiles. The volatiles from star anise, ginger and so on could extend the pre-oviposition period of PFB female adults. The volatiles from star anise, ginger and prickly ash had obvious affect on PFB adult longevity.2. Trans-anethole made the main role in the effects on hatching and the fumigation, contact toxicity, oviposition deterrence to the adults, but the efffects on the mature larva were not so obvious.The dead rate of the Carposina sasakii Matsmura treated by trans-anethole at different concentrations was 20.88～82.05%. Compared to the control group, the experiment showed high concentration made a large effect,while the low did not.The fumigation and contact toxicity test showed the contact toxicity rates of 10,20 and 50 times liquid was 100%. LC50 was 1396.06 times liquid. LC50 was 717.13 times liquid in fumigation test. The oviposition deterrence test showed 10 times liquid would fumigate the PFB to death. The oviposition deterrent rate of 20 times liquid was up to 94.13% and the rate of 1000 times liquid was only 5.16%. The statistical result of the mating rate showed 20 times liuquid was 36.67% and 1000 times liquid was 80%,which were not quite different from the control. 3. The effects of trans-anethole on the content of haemolymph protein: Test the change of content of haemolymph protein in the Carposina sasakii Matsmura treated with trans-anethole by the measure of spectrophotom. The result showed the content of haemolymph protein did not change obviously during the Initial Stages.60 min later, it began to increase largely and achieve the max. when it is 80 min. Then the content decreased and finally it was lower than control group,the haemolymph protein in which did not change much.4. The effects of trans-anethole on the protective enzyme: The activities of superoxide dismutase(SOD),peroxidase(POD)and catalase (CAT) did not change so much in the Carposina sasakii Matsmura treated by trans-anethole.The activities of superoxide dismutase(SOD),The activities of POD and CAT increased rapidly after 60min and decreased in different degree during the Later Stage, while the POD increased,higher than the control group. in the same time. Generally, the changing trends of the former two were the same and the POD increased steadily.5. The effects of trans-anethole on the detoxifying enzyme: All the detoxifying enzymes would be effected by the trans-anethole in different degree. The activity of glutathione-S-transferases(GSTs)was firstly activated,then inhibited ,and finally activated; Acetylcholinesterase(AchE) was firstly activated and finally inhibited. |
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