| Test one: Pelteobagrus fulvidraco, initial mean weights were (13.82±1.06) g, as thesubject of this experiment. In the mode of practical feed formulation, the contents ofprotein, fat, energy and phosphorus in feed are basically the same. We selectedcommodity pigment additives and the feed raw materials containing large amount oflutein, such as corn gluten meal as the composition of feed, to carry out the feedingexperiment simultaneously. We used high pigment content of feed ingredients tocompose the feed directly, to ensure the needs of pigment demand, and reduced the useof soybean meal and other plant protein at the same protein level. We hope to filter outthe appropriate adding amount of corn gluten meal, and to explore the feasibility ofreducing or none lutein additives. The test fishes were randomly assigned to five groupswith three parallels, and100fishes with each parallel. Increasing the amount of corngluten meal in feed, none adding of corn gluten meal was control group, and the addingof golden prime-Y commodity pigment additive was lutein group.58days after feeding,assay of the indicators of growth and body, feed efficiency, serum biochemicalparameters, immune function, the pigment content in skin and serum, and intestinal andliver tissue structure. The results showed that:①In the corn gluten meal adding group,with the increase of corn gluten meal, the feed conversion ratio showed an increasingtendency, specific growth rate was decreased, fatness was a decreasing trend.②TheAST, ALT of serum of6%corn gluten meal group were significantly higher than luteingroup.③In lutein group, the content of total carotenoid in serum was highest,significantly higher than that in control group and6%corn gluten meal group, while incontrol group, the content of total carotenoid in serum was lowest.④The height ofintestinal villus of various groups differed significantly (P <0.05). The density ofintestinal villus of6%corn gluten meal group was significantly higher than the10%corn gluten meal group and14%corn gluten meal group. Conclusions:①The results of growth performance: Considering the growth, survival rate and other factors ofPelteobagrus fulvidraco,6%corn gluten meal group was best.②The results of bodycolor effects: The content of total carotenoid and lutein in feed, serum and skin ofPelteobagrus fulvidraco showed the relationship of positive correlation. The content oftotal carotenoids and lutein in serum, abdominal skin and back skin of Pelteobagrusfulvidraco was cubic curve variation with that in feed. As a raw material of endogenousfeed pigment, corn gluten meal had a certain effect to maintain the normal body colourof Pelteobagrus fulvidraco. The coloring effect of lutein adding group with high contentof lutein was better than that of corn gluten meal adding group. With the increasing ofthe amount of corn gluten meal, the deposition of carotenoids and lutein in the skin ofPelteobagrus fulvidraco gradually increased, and10%corn gluten meal group and14%corn gluten meal group obtained better coloring effect as well.③The results ofphysiological function: No significant effect on the physiological function ofPelteobagrus fulvidraco with the increase of corn gluten meal.④The results of tissuestructure: The height and density of Microvilli were negatively correlated to the amountof corn gluten meal, however the permeability of intestinal was not significantdifference and the intestinal barrier of Pelteobagrus fulvidraco was integrity, and theliver of Pelteobagrus fulvidraco was not damaged.Test two: Pelteobagrus fulvidraco was the investigative subject of this experiment.In the mode of practical feed formulation, the contents of protein, fat, energy andphosphorus in feed are basically the same. Increasing the use of corn gluten meal mayaffect the body color and the growth rate of Pelteobagrus fulvidraco. Therefore, addingde-mold agent in feed and carrying on the confirmatory and effective test. The testfishes were randomly assigned to six groups with three parallels, and100fishes witheach parallel. The amount of corn gluten meal is set to6%,10%,14%, and we added0.1%mycofixr select in corn gluten meal group.58days after feeding, assay of theindicators of growth and body, feed efficiency, serum biochemical parameters, immunefunction, the pigment content in skin and serum, and intestinal and liver tissue structure.The results showed that:①The14%corn gluten meal group with mycofixr select hadsignificant reduction of feed coefficient (P <0.05), Adding mycofixr select in6%corngluten meal group and14%corn gluten meal group played a catalytic role on thegrowth of Pelteobagrus fulvidraco. The10%corn gluten meal group adding mycofixr select decreased the serum DAO significantly.②With the increase of corn gluten meal,the content of pigment in skin and serum of Pelteobagrus fulvidraco showed anincreasing trend.③The density of intestinal villi of mycofixr select adding group hadmore significant increasing than corn gluten meal adding group (P <0.05). The height ofintestinal villi of10%corn gluten meal group adding mycofixr select had moresignificant increasing (P<0.05). Conclusions:①The results ofgrowth performance: Thecontent of voitoxin in feed and fish muscle did not changed significantly. Mycofixrselect promoted the growth of Pelteobagrus fulvidraco. Considering the growth,survival rate and other factors of Pelteobagrus fulvidraco,6%corn gluten meal addingmycofixr select group was best.②The results of body color effect: The corn glutenmeal used in this experiment was mild fungal contaminated. The mycotoxin in corngluten meal had no significant effect on pigment deposition of Pelteobagrus fulvidraco.The content of total carotenoid and lutein in feed, serum and skin of Pelteobagrusfulvidraco showed the relationship of positive correlation. The content of totalcarotenoids and lutein in serum, abdominal skin and back skin of Pelteobagrusfulvidraco was cubic curve variation with that in feed. As a raw material of endogenousfeed pigment, corn gluten meal had a certain effect to maintain the normal body colourof Pelteobagrus fulvidraco.10%corn gluten meal group and14%corn gluten mealgroup obtained a better coloring effect than6%corn gluten meal group.③The resultsof physiological function: Under the experimental conditions, the mycotoxin had nosignificant affection to physiological function of Pelteobagrus fulvidraco.④The resultsof tissue structure: There was no liver injury trend in each group, nor the fatty liverdevelopment trend. The height and density of intestinal villus increased in varyingdegrees after adding mycofixr select. In each experimental group, the height and densityof intestinal villus of10%corn gluten meal adding mycofixr select group was highest. |
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