Ivestigation On Insect Pests Of Sophorajaponica L.Greening Trees And Study On It’s Genetic Diversity

Sophora japonica L easy to survive, particularly in the absorption of pollutants, air purification and so unique, as one of city greening tree species selection, is widely grown for street greening. However, this favorable environment green plants, but also to many pest colonization provides an excellent venue which has become a locust tree scale insect populations of the advantages of harm, and it easily with the seedlings and trees transporting, Long-distance spread in space, invade a new ecological system caused widespread harm to the agricultural and forestry production caused a great threat to security.First, this experiment in Taiyuan Yi Fen Street (more concentrated in young Ash), South inner (more concentrated in the age of Ash), Jinci Road (old acacia trees are concentrated) sample survey on the acacia trees, looking for different The scale insect species, and different niche on the number of scale insect, the type used for statistical analysis, after species identification, find out what the city is mainly affected by acacia trees against scale insects, vehicle exhaust, dust and other pollution Kuwana Would affect the distribution of insects. Finally, rapid, accurate and effective quarantine methods. According to our investigation, we have found great dates the Scale Eulecanium gigantea (Shinji), and fold the big ball firmly Eulecanium kuwanai Kanda locust tree in our province is the main type of hazard.Two, Genomic DNA were extracted from five scale insect by using the sodium dodecyl Sulfate (SDS) method, the cetylt ethylammonium bromide (CTAB) method, Phenol-chloroform (TES) method, the sodium chloride (NaC1)method and TNES method respectively. The results show that the five methods were useful in extraction of genomic DNA of scale insects. But comparatively speaking, the quality of DNA samples isolated by using the CTAB method and the TES method was obviously better than that by using the other three methods. So that, our laboratory think that the CTAB and the TES method were more effective and practical method for the genomic DNA extraction of scale insect.Three, The mitochondrial COI-COII sequences were of five scale insects were were amplified by using PCR technique to analyze their sequence variation and then design their respective specific primer sequence. The results show that, based on the molecular techniques of PCR amplification and direct sequencing can achieve accurate,rapid identification of five scale insect.