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Host Selection Mechanisms Of Apolygus Lucorum To Different Resistant Cotton Varieties(Lines)

Posted on:2013-09-17Degree:MasterType:Thesis
Country:ChinaCandidate:Y CaoFull Text:PDF
GTID:2233330374468330Subject:Agricultural Entomology and Pest Control
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This article uses cotton-volatiles-Heteroptera: Miridae as research model,Researchedselective preferences of Apolygus lucorum to cotton plants with different resistance levelsand damaged by Heteroptera: Miridae;Analysed the main element of volatiles of differentdifferent resistance levels and damaged by Heteroptera:Miridae; The major results are listedas follows:1. An experiment by visual survey and counting of insect population density wasperformed to identify and assess resistance of162cotton varieties to mirid bugs in cottonfield. The16varieties showing potential resistance to mirid bug were Asian cotton, Ling-29,and07Shengshi NO.6and others. On the other hand,6varieties were found to be potentiallysensitive to mirid bugs; there were Liaoyangduomaomian, Jifeng989, and Jifeng989,08shengshi NO.6, Daling668, Shikang338and HB5. Based on the experimental results a set ofprimary technical criteria for the identification and evaluation of field resistance in cottonvarieties to mirid bugs were established, they can be used as references in breeding cotton forresistance to mirid bugs.from all of breedings,we chose resistance varieties Ling-29and07shengshi No.6,sensitive varieties Kelin08-23and Kangbingdanzhu-1to followingresearch.2. Apolygus lucorum was divided by experienced and no-experienced.Using resistantvariety Ling-29and sensitive variety Kelin08-23as tested materials, resistant variety07shengshi NO.6and sensitive variety Kangbingdanzhu-1as tested materials seventreatments were selected including resistant variety,sensitive variety,resistant varietydamaged by Apolygus lucorum,sensitive variety damaged by A. lucorum, using fresh air asblank control, groups were conducted to investigate the taxis responses of A.lucorum tocotton plants with different resistance levels and damaged by Heteroptera: Miridae withY-tube olfactometer method.It was found resistant varieties damaged by A.lucorum,resistantvariety damaged by A. lucorum,blank control,respectively.In general, A. lucorum tended to significantly choose sensitive cotton varieties,and had an aversion to resisant cottonplants.A.lucorum tended to sensitive variety damaged by A. lucorum treatment andsensitive variety damaged by A.lucorum treatment.3. The volatiles of6different treatments of cotton were collected,GC-MS was used forqualitative and quantitative assay.The variety and the quantity of the volatiles on healthyresistant variety and sensitive variety had significant difference,we didn’t find n-Hexylacrylate,2,4-Dimethylstyrene, Benzaldehyde,4-ethyl, m-Ethylacetophenone, Ethanone,1-(4-ethylphenyl), Nonane,2,2,4,4,6,8,8-heptamethyl in resistance cotton varieties,butCumene exist in resistant variety exceptionally.Besides,on resistance cotton varieties,thevolatilize quantity of1,3-Diethylbenzene,1,4-Diethylbenzene, Naphthalene decreasedsignally. But the volatiles from the same kind of cotton damaged by different kinds ofHeteroptera: Miridae had no significant difference, only but individual volatiles havesignificant difference.It was also found that the quantities of cotton volatile of all of cottonvarieties were flowering>squarring> bolling> seedling.4. According to the result above,we selected15kinds of volatiles which has significantdifference:Hexyl Acrylate, Cumene,1,3-Diethylbenzene,1,4-Diethylbenzene,2,4-Dimethylstyrene,4-Ethylbenzaldehyde, Naphthalene,4′-Ethylacetophenone,2,2,4,4,6,8,8-Heptamethylnonan, P-Xylene,(1R)-(+)-α-Pinene, Camphene,Eucalyptol,Linalool, Butyl Acrylate.Mineral oil was treated as CK,Methyl phenylacetate was treated asCF,we tested EAG of antenna of male and female A.lucorum.The result showed that EAG ofantenna of A.lucorum had significant difference on15kinds of standard samples.The femaleto1,3-Diethylbenzene and Butyl Acrylate especially significant difference,EAG was exceedother standard samples significantly.But there was no significant difference between maleand female,except2,4-Dimethylstyrene.The other14kinds samples had no significantdifference.5. We used Y-tube olfactometer to ve refy the taxis responses of A.lucorum to15standard specimens indoor.The results shows,seven standard specimens:1,4-Diethylbenzene,Naphthalene,4′-Ethylacetophenone,2,2,4,4,6,8,8-Heptamethylnonane, P-Xylene,(1R)-(+)-α-Pinene,Camphene had no remarkable response.Two standard specimens hadremarkable attraction to A.lucorum:4-Ethylbenzaldehyde,1,3-Diethylbenzene.A.lucorumhad an aversion to six standard specimens:Cumene=Butyl Acrylate> Eucalyptol>2,4-Dimethylstyrene=Linalool>Hexyl Acrylate.The result above was essentially the same asthat EAG.
Keywords/Search Tags:Cotton, Apolygus lucorum, Plant volatiles, Y-tube olfactometer, GC-MS, EAG
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