Study On Four Antioxidants For Crypreservation Of Boar Spermatozoa

That to accelerate researching the conservation of boar frozen semen, has its ownsignificant point in animal husbandry development. Using boar frozen semen can improve theutilization rate of male animal, quicken the process of reproduction, and cut down the cost offeeding and administration. However, this method has been not utilized widely due to thecurrently lower quality and fertilizing rate of boar frozen-thawing semen. Numbers of studiesindicate that integrality of structure and function of boar sperm could be protect effectively ifadded antioxidant into frozen diluents to strengthen sperm quality after being frozen. Thisstudy mainly focused on four antioxidants, which contained bioflavanoid, Vitamin C and alsoE, and polyunsaturated fatty acid, to assay their protective effects on boar sperm, furthermoreprimarily explored those four antioxidants’ mechanisms in preventing damage caused by theprocess of freezing. The chief conclusions were as following.1. In the process of boar semen frozen conservation, both bioflavanoid and Vitamin Cadded into basic diluents could enhance quality of boar semen after freezing-thawing. Amongthose different levels of treatment groups,0.8mg/ml bioflavanoid and6mg/ml Vitamin Cperformed the best respectively. In order to access effects that the two combined to have, thisstudy also combined0.8mg/ml and1.0mg/ml bilflavanoid with4mg/ml and6mg/ml VitaminC respectively, then analyze Sperm viability, acrosome integrity, plasma membrane integrityand mitochondrial activity after freezing and thawing sperms. The results showed that thecombination of0.8mg/ml bilflavanoid with6mg/ml Vitamin C had much remarkable effectthan the control group (P<0.05).2. A great deal of studies have stated that both polyunsaturated fatty acid and Vitamin Ecan prevent fat from over-oxiding, and protect structure and function of cell membrane. Inthis study,1%,2%,4%,6%polyunsaturated fatty acid and0.2mg/ml,0.4mg/ml,0.6mg/ml,0.8mg/ml Vitamin E were respectively added into basic diluents to observe the roles thoseadditions played on frozen semen. The results showed that4%polyunsaturated fatty acid and0.6mg/ml Vitamin E played much significant roles than the control group had (P<0.05).Moreover, a further step was about to certain the compatibility effects. So4%,6%polyunsaturated fatty acid combined with0.4mg/ml,0.6mg/ml Vitamin E in various ways, which illustrated that the group of4%polyunsaturated fatty acid and0.6mg/ml Vitamin Eimproved the quality of frozen sperms (P<0.05).3. The concentrations of SOD, CAT and GSH-Px inside the boar sperm cells presenteddecreasing tendency during freezing. After equilibrium, compared groups treated withbioflavanoid, Vitamin C and also E, and polyunsaturated fatty acid with control group, thedifferences of three enzymes among those groups were not distinguished (P<0.05). Afterthawing, adding bioflavanoid Vitamin C and also C as a combination into basic diluentssplendidly relieved activity descents of above three enzymes, compared to control treatment(P<0.05). By adding four antioxidants separately, all could raise the activity of SOD and CAT(P<0.05), otherwise the GSH-Px could not give an obvious change.