Optimization Of Fermentation Strains And Preliminary Research Of Conditions In Solid-fermentation Of Rapeseed Meal

In this assay,Rapeseed Meal was obtained from Hubei COFCO(China Oil And Foodstuffs Corporation) as test meterial, microbial fermentation as test method, to optimize the ability of removing glucosinolate content and improve crude protein content.The research content were divided into four parts:i.Optimization of highefficiency vrius-free multi-strains;ii.Optimization of glucosinolate released fermentation process on response surface way;iii.Optimization of crude protein content increased fermentaion process on response surface way;iv.Optimization of the stockpiling fermentation process. The research results were as follows:i.Through solid-fermentation.for titer to the rate of glucosinolate released and crude protein content, we obtained five highefficiency vrius-free strains.Combination analysis of not less than three strains.we got that when multi-strains contain Bacillus subtilis S(ii),Bacillus natlo N(i).Bacillus laterosporus L(i),Bacillus cereus C(i) and Saccharomyces cerevisiae Y(i) on the same level.the conclusion was best,could to be the further study.ii.On the basis of single factor experiments, we got the main effect factors, then optimization of fermentation process parameters on response surface design.The results showed that when adding1.0%glucose.0.5%ammonium sulfate,70g Rapeseed Meal in250ml triangle bottle and initial pH6.0.it got fitted equation:Y1=16.7733-1.72326*A(inoculation amount)+2.76191*B(fermentation temperature)-1.35082*C(the ratio of material to water)-1.93787*D(fermentation time)+3.76996*A*A+7.18373*B*B+2.15588*C*C+5.64298*D*D-0.462875*A*B-0.426750*A*C-0.0259500*A*D-0.232325*B*C+0.204625*B*D-0.702750*C*D.When inoculation amount is7.6969%, the fermentation temperature44.1415centigrade, the ratio of material to water1to0.585350.the fermentation time52.6056hours, the glucosinolate content could down to15.8837μmol.g-1, the rate of glucosinolate released could reach up to85.0640%.iii.On the basis of single factor experiments, plus two orthogonal design experiments to confirm the main effect factors, then optimization of fermentation process parameters on response surface design. The results showed that when adding1.5%ammonium sulfate,0.25%glucose,9%inoculated capacity and initial pH6.0,it got fitted equation:Y2=43.3783+0.517667*A (load capacity)+0.490658*B (fermentation temperature)+0.608367*C (the ratio of material to water)+0.484508*D (fermentation time)-0.799996*A*A-1.54513*B*B-0.670971*C*C-1.23511*D*D+0.067650*A*B-0.699025*A*C-0.059275*A*D+0.356775*B*C+0.104250*B*D-0.0757500*C*D.When load is61.313g, the fermentation temperature40.7575centigrade, the ratio of material to water1to0.462625. the fermentation time97.8180hours, the crude protein content could reach up to49.4290%.was been increased8.8203%.iv.Basis on optimizational culture medium of glucosinolate released.it reach to a stockpiling fermentation medium.1.0%glucose.0.5ammonium sulfate.turning temperature is50to55centigrade.the ratio of material to water is1to0.60.the fermentation time is55to60hours after the temperature first up to44.1centigrade.v.Through fermentation.Amylase activity.Cellulase activity and Lipase activity of Rapeseed meal were improved on different levels.enhancement of total free amino acid is higher than8%.