Application Of Cotton Stalk Chip In The Substituted

Lentinula edodes is of important culinary and medical values. China, whose exports of dried mushroom account more than90%of that of the world, is the main producers and exporters of mushroom. Traditionally, the cultivation of mushroom primarily based on hardwood sawdust, which relies on a large consumption of forest resources. However, in our present day study, cotton stalk chip is selected as the substitute for woodchips as the basal ingredient of the substrate.We screen out the optimum substrate with cotton stalk chip as the basal ingredient by analyzing the effects of different formula which are of different ratios of C, N on the developmental growth, main agronomic traits and nutrients of fruit body with Q7and L607as the selected strains. The details are as follows:(1) Cotton straw and hardwood chips were mixed in different ratios, i.e. the contents of cotton straw are20%,40%,60%,80%and the contents of hardwood chips are60%,40%,20%,0respectively, they are numbered A、B、C、D. And the popular formula, numbered⑤with hardwood chips as the only ingredients is regarded as the control.(CK). The measurement of C, N, lignin, cellulose and hemicellulose of the five formula shows that the content of C,N, C/N, lignin, cellulose and hemicellulose in the four mixed substrates are46.22%～46.81%,0.78%-1.41%,32.86～59.80,26.31%-27.74%,24.68%-27.71%,13.53%～15.16%respectively. The C,C/N, lignin, cellulose and hemicellulose in the control are of the highest, with47.01%、81.78、28.12%、33.59%、15.94%respectively and N of the lowest is0.57%.(2) In the five aforementioned formla, Qiu7and L607were the selected strains and they were cultivated by adopting the method in which shiitake inoculated in autumn was placed in shelf during harvest period. As far as the cropping time is concerned, A,D of Q7, A,B,C,D of L607are significantly shorter than the control. The cropping time of the mixed substrate with the addition of cotton straw is reduced, however, as the increase the of ratio of cotton straw, the mycelium are in excessive growth,which is not conducive to the later development of the mycelium, due to the enlarged particle space, thus loosen structure of the bag. A、B of L607and Q7are cropped in a relatively concentrated time which extinct different flushes, the veil are mostly of middle size ranged from3cm to5cm. The biological efficiency of B in Q7and A、B in L607are76.74%,72.50%and73.05% respectively, which are higher than the controls with the one of Q7is66.02%and the one in L607is71.97%. The logs of C、D universally become soft after re-soaking in the later cultivation period. And the last flush of D suffered high contamination when encountered with high temperature. These two factor combined result in the decrease of biological efficiency of C, D.(3) The dry/wet, crude proteins, dietary fiber, Polysaccharide of the fruit body are measured. It reveals that dry/wet of these four formula of Q7and L607are1.43%～14.20%、12.19%～14.88%respectively. Variance analysis shows that both the dry/wet of the control of Q7and L607are of the highest level, A, B of Q7and A of L607when compared with the control respectively is insignificant. In the analysis of nutrients, the content of DF, crude protein, Polysaccharide and ash of A,B,C,D in Q7are38.67%-47.33%,13.89%-14.78%,3.43%-5.73%,5.0%-6.5%respectively. DF, crude protein of the fruit body which are cultivated from the control of Q7and L607are of the highest level with50.67%,15.00%and49.33%,13.56%respectively. Variance analysis shows that content of crude protein of B in Q7and L607are on the same level of the control, while the content of Polysaccharide is significantly higher.In conclusion, B is superior than the control when growth period, biological efficiency and certain nutrients of the fruit body are taken into consideration. Specifically, B has advantages of relatively shortened growth cycle, concentrated harvest time which are favorable for the effective management, highly accepted mushroom shape, superior nutrients contents, increased biological efficiency. To sum up, B deserves further promote.