Study On Genetic Diversity And Classification Among Waxy Corn Inbreed Lines In Shanxi

Waxy maize as the fresh maize is becoming more and more popular, and the market demands are also larger and larger. At present, the waxy maize inbred lines are often import farm waxy maizes to ordinary maizes by methods such as hybridization, backcross, etc., or directly use waxy maizes to hybridize and breeding, most of which are no pedigrees, and the genetic relationship between inbred lines is unclear. We can’t use genealogical tracing method to study genetic diversity, and combining ability test, isoenzyme marking and other methods also have some limitations. In recent years, the SSR molecular marker technology has been shown as an effective way to analyze the diversity of germplasm resources. Draw on the research methods of ordinary maize inbred lines, use SSR marker technology to research the genetic diversity of waxy maizes and guide waxy maize breeding, in order to reduce the blindness of hybridized combination in waxy maize breeding, and increase breeding efficiency and breeding level of waxy maize. This study use SSR molecular marker to analyze the genetic diversity of38waxy maize inbred lines, and construct DNA fingerprints for it, the results show that:1. The49couples of SSR primer covered the maize genome-wide all have polymorphisms in38waxy maize inbred lines, totally detected294alleles, alleles detected on different SSR locus are ranged from3to10, an average of6per locus. The polymorphism information content of each locus ranged from0.223to0.836, an average of0.633.2. It totally detected86rare alleles,32unique alleles. The locus of phi080existed most rare alleles (5), the bnlg1450and umc1018existed most unique alleles, and each has3. Zhu Xi waxy and Luo sticky rice separately contains5and4unique variances; Shanhei M and3B separately contain18and17rare alleles.3. According to SSR data, we can obtain the genetic similarity coefficients of38waxy maize inbred lines ranged between0.69and0.94. The minimum similarity number between inbred line05-34and B73,05-774and B73is0.69; the similarity number between inbred line L2and L4is0.94. While GS is approximately0.77, we divide waxy maize inbred lines into eight groups, the first group is inbred lines represented by Huangzaosi containing Tangsipingtou consanguinity, totally six parts; the second group is05-429, only1part; the third group is Luda Red group represented by Dan340, totally eight parts; the fourth group is Mo17represented by Lancaster group; the fifth group is the others group, totally23parts; the sixth group is the B73based on Reid; the seventh group is the PA group represented by Ye478, totally3parts; the eighth group is the PB group of Qi319.