The Study On Effects Of Methanol Stimulation On The Growth Of Faba Bean

The faba bean is less widely grown than soybean in plant protein resources in China, and its acreage expanded rapidly in recent years. Exogenous application of methanol (CH3OH) can enhance the growth of a variety of plants, this phenomenon should be positive in broad bean. But the enhancing effect of methanol (CH3OH) of plant growth is interrupted by a variety of external factors, then the exact mechanism is not clear, therefore researching about the mechanism of methanol to enhance the growth of faba bean help to reveal the methanol to enhance plant growth mechanisms, but also for methanol widely used in agriculture to provide a theoretical basis. We want to interpret molecular mechanism of methanol to enhance the growth of faba bean, obtained through experiments the following results:1. three weeks after spraying the10%,5%,2%concentration of methanol and water (control), we found the faba bean with spraying5%concentration of methanol in appearance, plant height, leaf size, leaf fresh weight were significantly higher than control and other plants of the spray concentration. Spraying5%concentration of methanol solution, the content of anthocyanin and the oxidative stress-related physiological indicators (MDA) has emerged as a significant downward trend, while PC and H2O2slightly elevated but did not reach significant values. These preliminary descript that the methanol can alleviate oxidative stress, anti-aging effect. Total chlorophyll, soluble sugar, total soluble protein content has emerged as a significant increasing trend, this preliminary evidence that the application of methanol can improve plant photosynthetic capacity and directly led to the increase of soluble sugar content. So far we can speculate that the physiological mechanism of methanol to enhance the growth of faba bean is to improve the net photosynthetic rate and carbon fixation ability, stimulate plant growth through photosynthesis-related physiological processes.2. Metabolic mechanism:13CHOH in the faba bean cell can first be transformed into H13CHO. H13CHO may formed adducts HM-GSH, then HM-GSH catalytic to formation of Formyl-GSH by FADLH. Formyl-GSH can be transformed H13COOH by FGH hydrolysis. Then part of H13COOH is oxidized to13CO2by FDH into the Calvin cycle and the major metabolite product was the [U-’’C] Gluc.. Some of [U-13C] Gluc was to be transformed into [U-13C] Fruc, but most of the [U-13C] Gluc can generate [2,3-13C] Ala by the glycolytic pathway, then [2,3-13C] Ala get into the TCA cycle transforming to carbon skeleton of other amino acid by forming [3-13C]Cit、Malate. We did not detect [2-13C]Ser of the signal peak in the faba bean, suggesting that [3-13C]Ser generation is by SHMT transforming of 13CH3OH oxidation production H13CHO which directly add with THF together to form5,10-13CH2-of THF.3. In order to investigate the mechanism of methanol to stimulate the growth of broad bean at the transcriptional level to build a5%methanol spraying Vicia the positive SSHcDNA library identified in Vicia145methanol-induced expression of genes, including95known genes for functional. Gene function in the cluster analysis results indicate that photosynthesis-related genes accounted for the largest proportion of45%。the library of gene expression by RT-PCR analysis showed its truth by that randomly selected gene, including the chloroplast protein complexes of light-harvesting chlorophyll a/b binding protein, plastocyanin, the iron-sulfur proteins, pectinesterase,chlorophyll-ray system protein, which expression is increased further in the library. Photosynthesis-related genes upregulated proof of methanol to promote plant photosynthesis. This may be the major molecular mechanism of methanol spraying to promote the growth of broad bean.