Comparison On Efficacy And Bacteriostasis Effects In Vitro Of Two New Dosage Forms Of Qingwenbaidu Powder

Along with the development of modern science and technology, many new techniques have been introduced into the production of Chinese herbal medicine(CHM). This not only makes dosage form of CHM become more abundant, but also improves the efficacy of CHM and facilitates clincal applicaton. Qingwenbaidu powder(QBP) is chinese patent medicine recorded in The People’s Republic of China Veterinary Pharmacopoeia. Its function is purging fire removing toxin, clearing heat cooling blood, and indication is pyretic toxicity spot poison, hyperpyrexia cona. It is commonly used to treat animal infectious diseases. The drug is a power made from crused raw material medicine. Recently, two new dosage forms,Qingwenbaidu granules(QBG) and Qingwenbaidu mixtures(QBM), have been developed. In order to evaluate the advantages and disadvantages of these two new dosage forms, this study compared the efficacy of two new dosage forms in artificially infected chicken E. coli, mixed infection of chicken E. coli and chicken pasteurella and the bacteriostasis in vitro. The purpose is to confirm the drug effects two new dosage forms and supply theoretical basis for exploiting new dosage forms and enhancing curative effect of QBP. The test included tree parts:Test1, Curative effects comparison of two new dosage forms of QBP for infection of chicken E. coli33930-day-old chiekens were randomly divided into9groups. The chiekens, except for the blank group, were artifically infected with Escherichia coli. then given different treatment. The chiekens in positive control group were not administered, in three dose groups of QBG wrer mixedly fed respectively with high-, midde-and low-dose QBG, in three dose groups of QBM were mixedly drunk respectively with high-, middle-and low-dose QBM, in QBP control group was mixedly fed with QBP, once a day for five successive days. The chiekens’clinical symptoms and death of each group were observed daily for7successive days. The death rate, cure rate, efficiency rate and weight gain of each group were compared. The results showed that the death rates of QBG middle dose group and QBM high dose group were significantly lower than that of positive control group(P<0.05), and cure rates and efficiency rates were significantly higher than that of positive control group(P<0.05). The death rates in high and middle dose groups of QBG and QBM were obviously lower than that in QBP control group, and cure rates and efficiency rates were obviously higher that in QBP control group. The average weight gain rates of QBG middle dose group and QBM high, middle dose groups were obviously higher than that in positive control group and QBP control group. The results indicated that thwo new dosage forms of QBP had reliable efficacy for chicken E. coli infection, QBG and QBM were superior to QBP.Test2, Curative effects comparison of two new dosage forms of QBP for mixed infection of chicken E. coli and chicken pasteurella36443-day-old chiekens were randomly divided into9groups. The chiekens except for the blank group were artifically infected with Escherichia coli and chicken pasteurella then given different treatment. The chiekens in positive control group was not administered, in QBG’s three dose groups were mixedly fed respectively with high-, midde-and low-dose QBG, QBM’s three dose groups were mixedly drunk respectively with high-, middle-and low-dose QBM, QBP control group was mixedly fed with QBP, twice a day for three consecutive days. The clinical symptoms and death of each group were observed daily for9continuous days to compared mortality, cure rate, efficiency rate, pathological change and weight gain situation. The results showed that in QBG’s middle and low dose groups and QBM’s three dose group, the death rates were significantly lower than that in positive control group(P<0.05), and cure rates and efficiency rates were significantly higher thaat in positive control group(P<0.05); In QBG’s low dose group, the death rate was significantly lower than that in QBP control group(P<0.05), and cure rates and efficiency rates were significantly higher than that in QBP control group(P<0.05); in QBM’s low dose group, the death rate was obviously lower than that in QBP control group, and cure rates and efficiency rates were obviously higher than that in QBP control group; The pathological changes in all of QBG and QBM group were significantly or obviously lighter than those in positive control group and QBP control group. The average weight gain rates of QBG’s high and middle dose group and QBM’s low dose group were significantly higher than those in positive control group and QBP control group(P<0.05). This indicated that two new dosage forms of QBP had reliable efficacy for mixed infection of chicken E. coli and chicken pasteurella, QBG and QBM were superior to QBP.Test3, Bacteriostasis effects comparison of two new dosage forms of QBP in vitro The bacteriostasis effects in vitro, minimal inhibitory concentration(MIC) and minimal bactericidal concentration(MBC) of QBG, QBM and QBP were determined for three kinds of chicken E. coli (O78, O18, O2) and two chicken pasteurella(C48-1, C48-2). The results showed that QBG was low sensitivity for five bacteria, and its MIC and MBC for three chicken E. coli were250and500mg·mL-1and for two pasteurella were125and1000mg·mL-1respectively. QBM was not sensitivity to five kinds of bacteria, and its MIC and MBC to three E. coli were500and2000mg·mL-1and to two pasteurella were250and500mg·mL-1respectively. The decoction of QBP was moderately sensitivity for pasteurella C48-1, low sensitivity for other four kinds of bacteria, and its MIC and MBC were same to QBG for the five kinds of bacteria. The results indicated that the bacteriostasis effects of QBG and QBM were not better than that of QBP decoction, the reason may be related to adjuvant in the preparation.