Effects Of The Specificity Antagonist For Miniature Pigs’Combined Anaesthetic On Monoamine Neurotransmitter And Endogenous Opioid Peptides In Anesthesia Rat’s Different Encephalic Regions

Based on balanced anesthesia theory and physiological characters of miniature pigs, combin-ed anaesthetic for miniature pigs (XFM), was succeeded manufactured. In order to ensure the con-trollability of the XFM and quickly palinesthesia after anesthetized with XFM, anesthesia group in Northeast Agricultural University had developed specificity antagonist for miniature pigs’combi-ned anaesthetic successfully by quadrature experimental and screening the best combinations. Aft-er accomplished the research of signal transduction mechanism and hemodynamics, this study was aimed to further discuss the antagonism mechanism from the perspective of neurotransmitter.In this experiment, making SD rat as the object and the specificity antagonist for miniature pigs’combined anaesthetic as the tested drugs, we inject the specificity antagonist for miniature pigs’combined anaesthetic in rat after anesthetized with XFM, at the time of nonage (10min after anesthesia), metaphase (30min after anesthesia), and advanced stage (60min after anesthesia).Then the sample of brain tissue were collected and determined respectively using high performance liquid chromatography (HPLC) and ELISA about monoamine neurotransmitter and endogenous opioid peptides. So we can investigate the antagonism mechanism from the perspective of neurotransmitter. Experimental results were such as follows:1.After intraperitoneal injection of XFM in rat, we carry out waking up the rat with intraperitoneal injection of the specificity antagonist for miniature pigs’combined anaesthetic. Then contents of NE and DA in rat’s different encephalic regions rised at the righting reflex recovery immediately, and decreased when rectilinear creeping. Contents of NE and DA in experiment group were higher than control group. Content of5-HT in different encephalic regions decreased.2.After intraperitoneal injection of XFM in rat, we carry out waking up the rat with intraperitoneal injection of the specificity antagonist for miniature pigs’ combined anaesthetic. Contents of endogenous opioid peptides in wake-up groups rised and wake-up groups’contents of β-EP in hippocampus, thalamus, brainstem were lower than control groups. There was no significant difference between wake-up groups and control groups. Wake-up groups’ contents of L-ENK, M-ENK and DYN in different encephalic regions were lower than control groups.3.With the method of detecting three monoamine neurotransmitters, there were high sensitivity and coefficient of recovery,94.75%,92.66%,92.50%respectively. And the coefficient of variability in innerday and daytime were lower than5%.Results showed that in the process of waking up rats, changes in contents of NE, DA in different encephalic regions shows an increasing trend. So the rising in contents of NE, DA were due to the specificity antagonist for miniature pigs’combined anaesthetic, and it reveals that NE, DA recovers excitability neural transmission, ensures the specificity antagonist for miniature pigs’ combined anaesthetic could reverse the anesthesia effects of XFM, and complete the wake-up. Maybe this is one reason of the antagonism mechanism of the specificity antagonist for miniature pigs’combined anaesthetic.After intraperitoneal injection of the specificity antagonist for miniature pigs’combined anaesthetic, contents of5-HT, DA present an decreasing trend. This result was consistent with the change of praxiology in rat. It shows that5-HT, DA play an important role in the progress of rat’s waking-up. Decreasing in content of5-HT reduced the inhibition of the central nervous system and this may be one reason of the antagonism mechanism of the specificity antagonist for miniature pigs’combined anaesthetic.After intraperitoneal injection of the specificity antagonist for miniature pigs’combined anaesthetic, contents of L-ENK, M-ENK, DYN, β-EP presented an decreasing trend. According to endorphin’s physiological action, decreasing in contents of L-ENK, M-ENK, DYN was relevant with disappear of analgesia. Decreasing in contents of β-EP may be due to the specificity antagonist for miniature pigs’combined anaesthetic competitively inhibit opioid binding with receptor. Due to releasing of β-EP in the central nervous system, content of (3-EP within the brain decreased, so that relieved the inhibition of endorphins on the central nervous and promoted or accelerated the awakening. This may be one reason of the antagonism mechanism of the specificity antagonist for miniature pigs’combined anaesthetic.