Preliminary Study On Pharmacognosy Of Dendrobium Officinale Kimura Et Migo Somatic Cell Asexual Variation

Objective:Dendrobium officinale Kimura et Migo is the orchid Dendrobium genus, it is the extremely high medicinal value of Chinese medicinal materials. Because of the wild resource scarcity, artificial planting area expanded unceasingly, and wild provenance and seedling cultivation sources complicated, the artificially cultivated kinds were changed. These variants from the plant to the internal structure and from components to taste are obviously different. It brought confusion and difficulty even development risk, for the sustainable development and utilization of Dendrobium officinale Kimura et Migo. This topic is to explore the differences between the cultivated variants Pharmacopoeia records Dendrobium officinale Kimura et Migo standard, For the screening of high-quality, high-yielding Dendrobium officinale Kimura et Migo artificial cultivars provide a variety of resources and technical data, in order to achieve sustainable development and utilization of the resources of Dendrobium officinale Kimura et Migo.Method:The subject use research of the pharmacognosy, the research methods of molecular biology techniques, analytical Dendrobium officinale Kimura et Migo artificial cultivation of variant and wild species of Dendrobium officinale Kimura et Migo the factor and different.Resultst:①The artificial cultivation of Dendrobium officinale Kimura et Migo variant difference in morphological characteristics and the morphological characteristics described by the Pharmacopoeia, fiber and starch grains with less.②The polysaccharide content:22.64%～49.16%.③Different methods of measuring differences in the content of mannose.④Molecular genetic similarity coefficient differences Conclusion:Through various means on the artificial cultivation of Dendrobium officinale Kimura et Migo variants, explore the recorded differences between the standard of Dendrobium officinale Kimura et Migo and Pharmacopoeia, get the technical data of the artificial cultivation of high quality, high-yielding Dendrobium officinale Kimura et Migo varieties.