The Polymorphisms Of THRSP Gene Associated With Lipogenesis And Ecological Factors In Goat Breeds

To understand the association of THRSP gene with habitats and fatty deposits in goatbreeds, the polymorphisms of goat THRSP gene, the fat content of goat carcass and theirassociation with the ecological factors were investigated. The Chinese indigenous goatbreeds selected were Guishan, Yunling, Chuandong, Banjiao, Xiangdong, Matou and Anbai.While the imported goat breeds were Boer goat and Sann Goat.The polymorphisms in the5’regulatory region and exon1ofTHRSP gene were analyzed using PCR-RFLP.The longissimus muscle fat grade (MFG) and carcass fat grade (CFG) of all goatbreeds were estimated. The observed data of ecological factors were provided by themeteorological observatory in goat habitats, which included average daily rainfall (ADR),elevation (E), average daily sunshine duration (DSD), latitude (LAT) and longitude. E/LAT,ADR×DSD and SC×EC were regarded as space coefficient (SC), energy coefficient (EC)and ecological index (EI), respectively. The results as follows:1. Three mutational sites, C-233T, G113A and A138G, were detected. Sequence analysis ofthe5’ regμLatory region showed that the homology between goat and cattle was97%, andthat between goat and human was80%.However, the homology between goat and cattlewas95%, while goat and pig was88%in exon1. The potential promoter region predictedlocated in-81~-32bp upstream of the start codon (USC) of the THRSP gene. In the5’regulatory region, there are nine E-boxes, one CCAAT box and two ATTGG boxes.C-233T site was NF-κB transcription factors binding site. The mutation at A138G sitecaused the change of amino acids from arginine to glutamic at38th site of THPSP protein.2. Nine genotypes in all goat breeds were only found, of which the frequencies ofTCAAGG, TTGAAG and TTAAGG genotype were lower than0.05. The genetic distancebetween Xiangdong and other goats was farthest, and that among Guishan, Yunling andMatou were nearer.3. The EC significantly related to CAG haplotype frequency (0.8859, P<0.01), and theassociations of EI with TGA haplotype frequency was significant (-0.8377, P<0.025). Inthe four haplotype frequencies, the relationship between CAG haplotype frequency andMFG was significant (-0.8633, P<0.025).