The Germplasm Resources Diversity Of Pleurotus Citrinopileatus

In this study,20strains of Pleurotus citrinopileatus were used as material for resource diversity by using methods such as the way of boanty、 isozyme techniques、 ISSR analysis.The results are as following:1. Morphological development of diversity of Pleurotus citrinopileatusThe Pleurotus citrinopileatus from spores to the ejection spores of the mature fruiting body needed74days.The different strains of development and internal structure were no obvious difference, the morphological development process was basidiospore germination,primary hyphae formation,secondary hyphae formation,the original base formation,finally developed into a complete mature fruit body.But the different types of basidiospore size,hyphae diameter,fruit body size vary slightly.The average size of spores of Pleurotus citrinopileatus (length×width) were5.66-8.25×2.06-3.38μm.The length of No.3spore was8.25μm,the width of No.12,13spores were3.38μm.To observe of the spores,their shape was a long oval, one end jagged, smooth surface, contain a nucleus;to observe by scanning electron microscopy, the spore was smooth surface which was to the side of depression, the results showed that the moisture of spores gradually reduced by drying.The average time is about25d from spore germination to primordia. Spore germination is from one end,also have started from both germination or other parts germination. Cultured for40hours, the short stick of mycelium gradually formed primary hyphae and branching hyphae appeared.About48hours.the diameter of primary hyphae was to3.62μm without clamp connection. After4～5days, two primary mycelia were fused by means of other parts and formed secondary mycelium.At least23days, the primordium formed.The culture time of hyphae needed7～10days. Both primary and secondary hyphae had diaphragm and branches, but the secondary mycelium and primary mycelium weight of thickness was not clear difference; primary mycelium had a nucleus between two diaphragm, secondary hyphae had two nuclei between two diaphragm and clamp connection.From the primordium to mature fruiting bodies required about5days.The internal morphological development were all primordia, the stipe primordium and pileus primordium gradually formed, the pileus mycelium and stipe mycelium formed arc which is not closed, fence-like cells arranged in a curved internal surface area, the formation of gill be here. The reproductive hyphae existed in the bacterial context of mature fruiting body, its diameter is usually1.63～3.88μm and clamp connection.The reproductive hyphae constituded the surface of cap.The longitudinal section of gill was wedge, hymenium covered on both sides of gill which was called the type of equation hymenium.The cap epidermis of20strains of mature fruiting bodies to two gill cavity were193.6～503.5μm, the cap epidermis than300μm varieties were P4,P10,P12and P16.The hymenium thickness was110.1～184.5μm, hymenium thickness was184.5μm whichwasP2,less than147.6μm was fifteen strainsThe length of burden stems were14.7-24.0μm, the length of burden stems of P2strain were24.0μm;less than19.3μm varieties were P1、 P3、 P6、 P8、 P11、 P12、 P15, P16P18、P19�'�P20.The various varieties of cap thickness, the thickness of the hymenium, the burden stem length were different,they were affected by environmental conditions.2. Studies on esterase isozyme analysis diversity of Pleurotus CitrinopileatusThe esterase isozymes of20strains of Pleurotus citrinopileatus were studied, the polymorphism was78.6%, by clustering analysis results could be seen, at the similarity level0.8the test strains were classified into seven groups, the first group included strains No.1,15,18,the second group included strains No.2,19,20,the third group included strains No.11, the fourth group included strains No.9, the fifth group included strains No.3,4,5,6,8,10,16,17, the sixth group included strains No.7,12,14, the seventh group included strains No.13.Combined with the morphological research results could be seen, the fifth group strains was larger than the other groups.The hybrid strains werel4,15,16,17,and there was the same parent strains of the source which had similar enzymes.3. Genetic polymorphism analysts of Pleurotus Citrinopileatus by ISSR1SSR method was used to analyze genetic polymorphism of20strains of Pleurotus Citrinopileatus strains, the results showed that the five primer amplification out64sites,it was the total of88.9%,20strains of Pleurotus citrinopileatus were rich genetic diversity.By UPGMA cluster analysi.s,at the similarity level0.68the test strains were classified into six groups.most of the strains from the same area could be clustered into one class.some cultivated strains and other areas could be clustered into one class, the phenomenon may be related to the frequent introduction. and ISSR can be effectively distinguish varieties for strain selection and preservation foundation.