The Role Of Forsythaside On The Cytokine And Antionxidative Function In Thymus Tissue Of Chickens

Endotoxinis the main component of the cell wall’s ectoblast of Gram-negative bacteria．Thechemical nature of endotoxin is lipopolysaccharide (LPS)．It can induce the inflammatoryreaction, and may induce the production of free radical, aggravate the damage of cell. The thymuswas important central immune organ in animals. Therefore, it become an important organ to testfor LPS induced body injury.Forsythia is a traditional medicine of China, the clinical application is very extensive,curative effect and side effect is low, and the biological activities are various.Forsythiaside has antibacterial, antiviral, antioxidant and other biological activity, in recent yearsmany scholars to study it, but before it didn’t be used to treat gram-negative bacteria induced bybacterial diseases of poultry. We study of the forsythiaside antibacterial, antioxidant andLPS-induced AA broilers thymus tissue oxidative damage.Therefore, this study was conducted to investigate the effects of forsythiaside on acutethymus tissue inflammation in chicken thymus tissue challenged with LPS and discussed thepossible mechanism, to provide theoretical basis for the further research and clinical application offorsythiaside. AA broilers were used as experimental animals and divided into six groups (10animals each). They are the normal control group, LPS control group, the low and high offorsythiaside control groups (30mg/kg,60mg/kg), the low and high of forsythiaside test groups(30mg/kg,60mg/kg).Orally administer forsythiaside from15d to21d. At21days of age, forsythiaside treatmentgroup and LPS control group chickens were injected with LPS at200μg/kg of BW. At2hpost-LPS injection, chickens were been killed for collecting thymus tissue. Weighing the weight ofthe thymus and measured the thymus index. Then, we compared the concentrations and mRNAexpression of interleukin (IL)-1β, IL-6, IL-17and TNF-α in the thymus tissue, using ELISA andReal-Time PCR.The test results are shown, Injection with LPS increased (P<0.05) the content of IL-1β, IL-6and IL-17compared with the control group. The groups of therapy by forsythiaside (30,60mg/kg)decreased (P<0.05) the protein and mRNA levels of IL-1β, IL-6, TNF-α and IL-17comparedLPS control group.These results suggest that oral administration of forsythiaside mitigated thethymus tissue inflammatory response induced by LPS through changes in the production ofpro-inflammatory cytokines such as IL-17, IL-1β, IL-6, and iNOS. Detection of total antioxidant capacity (T-AOC), superoxide dismutase (SOD),malondialdehyde (MDA), and Glutathione (GSH) content in thymus tissue. Compared with thenormal group, forsythiaside control groups T-SOD, T-AOC and GSH contents elevate obviously,proved that forsythiaside has the antioxidation effect. Compared with the LPS group, in theforsythiaside treatment groups T-AOC, T-SOD and GSH content elevates obviously, the MDAcontent markedly reduces, explained that the forsythiaside can promote T-AOC, T-SOD and GSHactivates, inhibition of MDA, thus reducing tissue damage.In one word, the study shows that forsythiaside inhibits significantly LPS-induced thymustissue inflammatory responses in chickens, and it can to improve the change of LPS-induced AAchicken thymus tissue antioxidant function. Some of its relationship between inflammatorymediators and antioxidant are expounded, to provide the theoretical basis for the further research.