Chinese Forest Frog Eggs Oil Related Gene Expression Studies In Hyperlipidemia Rabbit

The Oviducts of forest frog has the effect of reducing platelet adhesion, scavenging freeradical, regulating blood lipids, reducing thrombosis delay menopause, and it is a new green healthproducts. According to the composition of the Oviducts of forest frog, in view of the high incidenceof high cholesterol patients, through this study, rabbit fed with different months of different dosesof the Oviducts of forest frog, determine the efficacy of the optimal dose of play; while usingReal time PCR technology of ApoE gene, LDL-R LDL-R gene, ABCA1gene in rabbit spleentissue expression pattern of time and space, suggesting the possibility of Oviducts of forest frogmolecules play a pharmacodynamics mechanism. The main conclusions are following:1. Health-care group,Continuous lavage the Oviducts of forest frog within40days, the amountof LDL-R gene expression in the spleen and the liver tissue increased in different degrees.health-care group with gastric lavage3.25g/kg,Continuous lavage30days spleen organization inthe largest amount ofApoE gene expression. Within30days of model group, the level of ApoEgene expression was in different degree of elevated in the lipid-lowering group and intensivelipid-lowering group.Quantity On the whole, the lipid-lowering group and intensive lipid-loweringgroup with gastric lavage forest frog oil3.25g/kg,Continuous lavage20days,had the largestamount ofApoE gene expression.2. health-care group,Continuous lavage the Oviducts of forest frog within40days, theamount of LDL-R gene expression in the spleen and the liver tissue increased in different degrees.3.25g/kg Continuous lavage20days spleen organization had the largest amount ofApoE geneexpression. Model group within30days of continuous intragastric the Oviducts of forest frog, thelipid-lowering group and intensive lipid-lowering group spleen tissue ABCA1gene expression wasin different degree of elevated. Quantity On the whole, the lipid-lowering group with gastric lavageforest frog oil3.25g/kg,Continuous lavage20days,has the largest amount of LDL-Rgeneexpression,compared with controls extremely significant difference （P<0.01）, intensivelipid-lowering group was not significant (P <0.05)3. health-care group continued orally within40days ABCA1gene expression increased indifferent degrees.3.25g/kg Continuous lavage30days spleen and the liver organization had thelargest amount of ABCA1gene expression. model group within30days of continuous intragastricdose, the lipid-lowering group and intensive lipid-lowering group spleen tissue ABCA1geneexpression was in different degrees of elevated. Quantity On the whole, the lipid-lowering groupand intensive lipid-lowering group with gastric lavage3.25g/kg,Continuous lavage20days,had the largest amount of ABCA1gene expression,compared with controls extremely significant difference（P<0.01）, intensive lipid-lowering group was significant difference (P <0.05)4. The Oviducts of forest frog could make ApoE, LDL-R ABCA1gene expression of thehealth-care group and model group significantly increased, the LDL-R gene expression ofhealth-care group was on the largest,with least impact on the ABCA1gene. the ABCA1geneexpression of the model group is on the largest,with least impact on theApoE gene. The frog egg oilin rabbit liver tissue ApoE LDL-R, ABCA1gene expression were higher than the amount ofexpression in the spleen.5. The Oviducts of forest frog Regulated ApoE, LDL-R, ABCA1gene expression in theprocess of lowering blood pressure,with lipid-related genes in the regulation ofthe Oviducts of forest frog can play the role of its lipid-lowering, And3.25g/kg dose is the bestconcentration. Taking the Oviducts of forest frog regularly with health care function, has a certaineffect on the prevention of hyperlipidemia,3.25g/kg dose rabbits with hyperlipidemia preventionconcentration,3.25g/kg dose is the most effective treatment of rabbits with hyperlipidemia.