Lily Hybrids F1Generation Of Genetic Variation Character Analysis

Lily （Lilium） is a famous flowers, occupies an important position in international cut-flower production, Rich germplasm resources, long history of cultivation, It is rich in design, design and color is gorgeous, cultivation is extensive, can be used for cut flower decoration, garden greening, and the lily of the bulb is edible and medicinal value. This studythrough conventional hybridization breeding of hybrid seedlings, After through field statistics,choose colour is beautiful and flower type is special from Seven hybrid combinationsF1generation155samples of materials,carry through Inter-simple sequence repeat,By hybrid genome amplification marked results,Calculation of genetic similarity coefficient of parentand offspring，Proportion of polymorpHic loci,In order to illustrate children and parents genetic similarities，Reasonable to assess the validity of hybrid.in addition, the hybrid F1generation materials in flowering period was14morphological traits index determination,through analysis of variance, correlation and principal component analysis method to analyze14indexes is order to select excellent cross combinations and advantage hybrids materials.This study made the following main results.（1） After field management, choose Special varieties of designs and colors from Sevenhybrid offspring，in addition, Choose the best plant more plants from A₆×A₅hybridized combination，the Perianth color is pink，One for the gold，Shallow peachblossom edge series,Th-ere are flowers golden yellow in color,Shallow peachblossom edge series. Another series of perianth color for yellow, dark pink edge.（2） For seven lily parents and hybrid F1generation of155materials for related research,Selected7primers they are amplify clear belt type, and good polymorphic from99primer,the molecular weight between100-2000bp, The polymorphisms of the stripe number is171, the polymorphic rate was67.32%. Article24.43per primer amplification DNA fragments, The amplification polymorphism loci percentage difference is obvious, The maximumis0.85, the minimum is0.33,Other primer amplification loci in hybrid combinations similar between0.50.8.（3） In A₃×A₄hybrid combinations at Seven hybrid combinations, Based on the data of22selected ISSR materials for cluster analysis，we Can be analyzed from the tree diagram：At thethreshold of0.49，The selected material can be divided into two broad categories，the first speciesincluding2material,Showed that the individuals were close genetic relationship in this group, the second species including20material and male parent was included, Showed that the individuals inthis group and male parent close genetic relationship,the others19single plant tend to maleparent,the largest genetic similarity coefficient is0.6666,General tendency to female parent beforethey tend to be male parent.（4） The experiment analyzed the genetic diversity of characters of F1generation of AsiaticHybrids ‘Dark Beauty’ and ‘Pollyanna’ and their parental generation, and carried out thecorrelation analysis and principal components analysis of F1generation. The results show thatthere are significant differences, at the level of1%, between F1generation and their parentalgeneration in several characters: plant height, bulb circumference, leaf length, leaf width, insideround perianth length, anther length, anther width, filament length, style length, and bud number.The level of differences of corolla diameter between theF1generation and their female parent andmale parent are1%and5%, respectively. Although, the differences in outer perianth length andwidth between them are not significant, appearing a good hybrid advantages overall...