Influence Of Vaccaria Segetalis On Cellular Signal Transduction Pathway Associated With Lactation In Dairy Cow Mammary Gland Epithelial Cells

Low milk yield, low milk fat percentage and protein content and high incidence of mastitisare serious problems in Chinese dairy industry, which are the important factors limit thedevelopment of dairy industry of our country. So, how to improve the quality of milk efficientlyand safely has became an important problem of dairy industry. The study of the influence oncellular signal transduction pathway associated with lactation in dairy cow by Chinese medicineand other natural active substances could provide theoretical base and practical guidance inimproving of milk production and milk quality in dairy cow, which has extremely important valueof theory research and application.It has been confirmed that the Chinese medicine Vaccaria segetalis has the characteristics oflactation of female animal and, has been isolated biological active components, such as cyclicpeptide, flavonoid glycosides, triterpenoid saponins, fatty acids, lipids and sugars and so on fromVaccaria segetalis, which provide important information for the research of biological mechanismof Vaccaria segetalis, but how it to regulate the cellular signal transduction pathway associatedwith lactation of dairy cow mammary gland epithelial cells (DCMECs) and promote lactation isstill unknow. The aim of this work is to provide scientific theory for development of Vaccariasegetalis and also provide an important basis for the development and utilization of wildliferesources of Vaccaria segetalis through studying the influence of cellular signal transductionpathway associated with lactation of DCMECs in vitro by Vaccaria segetalis.Our work was divided into two parts. The fist part as follows: water extraction of Vaccariasegetalis was prepared, CASY was used to study the cell viability and proliferation treated with,estrogen (ER), prolactin (PRL) and Vaccaria segetalis; western blotting was used to detect thechanges of signal molecules associated with lactation in DCMECs treated with, estrogen (ER),prolactin (PRL) and Vaccaria segetalis; the second part as follows:cells were stimulated by addingmethionine, methionine and Vaccaria segetalis, methionine and PRL, methionine and E,respectively. The addition of sodium acetate as follows: cells were stimulated by adding sodiumacetate, sodium acetate and Vaccaria segetalis, sodium acetate and PRL, sodium acetate and E.CASY was used to study the cell viability and proliferation; Western blotting was used to detect the changes of signal molecules associated with lactation in DCMECs. The results showed that:1) Vaccaria segetalis could promote the viability and proliferation of DCMECs (P<0.05), theexpression of MAPK and cyclinD1were higher than control;2) Vaccariae segetalis could regulates and activates E and PRL signaling pathway. Theexepression level of S6K1, AKT1, p-mTOR, p-STAT5, p100, GARS, PRLR and ERα wereextremely higher than control (P<0.05);3) Vaccariae segetalis had no apparent effects on signal molecules associated with milk fatsynthesis. The expression of PPARγ was not extremely changed by Vaccariae segetalis;4) Methionine could promote the viability and proliferation of DCMECs (P<0.05).Methionine could regulate the signal molecules associated with protein synthesis,the expression ofGARS and p100were up-regulated (P<0.05);5) Sodium acetate had no apparent effects on viability and multiplication capacity ofDCMECs. Sodium acetate had no apparent effects on the expression of p100, GARS, PRLR andERα.