The Effect Of The Inhibitor Of Src On The Biological Behaviours Of Human Gastric Carcinoma Cells

Objective Src is the first discovered human oncogene, which have tyrosine kinase activity. Its clinical value has attracted a wide spread attention. In this study, we investigated the effect of the inhibitor of Src - pp2 on the growth, motility, invasion and angiogenesis of human gastric carcinoma cell line SGC - 7901. Methods Stable expression of Src and p -Src proteins was confirmed by western blot analysis Src and p - Src protein expression were tested by Immunohistochemistry; We measured effect of pp2 on proliferation p - Src protein expression of human gastric carcinoma cell line SGC - 7901 by Western blot; MTT was used to evaluate cell proliferation. Tumor invasion was examined by Transwell method, and tumor cell migration by wound - healing method. Results Immunohistochemistry revealed that Src and p - Src were expressed in the cytosol in the human gastric carcinoma cells. Western blot analysis showed that 5μmol/L pp2 had marked inhibitory effect on p - Src expression in the human gastric carcinoma cell line SGC~ 7901. When Human Gastric Carcinoma SGC~ 7901 wrere cultured with pp2 for 12 hour, the result showed that pp2 could not impact on cell proliferation at 0μmol/L(0. 33±0. 22), 5μmol/L (0. 33±0. 21) and 10 limol/L (0.33±1.02) (P>0.05), while 15 ^mol/L (3.63±1. 02) could inhibit cell prolif-eration(P<0. 05); In the membrance invasion culture system (Transwell chamber), suppressions were noted after pretreatments with pp2(5μmol/L and 10μmol/L), this effect can be enhance by the concentration gradually increased, the migration distance was (3. 92±0. 13)μm, (2. 54±0.16)μm and (1.56±0.12)μm at 0μmol/L, 5μmol/L and 10μmol/L, respectively. There were more cells invading through thematrigel in 5μmol/L and 10μmol/ L group than that in 0μmol/L group in a concentration - dependent manner [ (321. 67±28. 39) and (256.00±29.84) vs (496. 67±34.64), P< 0.05]. Angiogenesis in vitro assay showed that pp2 could inhibit the angiogenesis of human gastric carcinoma cell line at 5μmol/L and 10μmol/L compare with 0μmol/L group[ (17.33±1. 89) and (11.17±1. 49) vs( 24.5±1.71), P< 0.05 ]. Conclusions Pp2 could inhibit the growth, invasion, metastasis and angiogenesis of human gastric carcinoma cell line SGC - 7901. Src has the potential to become a new target for treatment of gastric carcinoma.