| Objective: Through detecting APM1gene’s four single nucleotide polymorphic sites(SNPs) in genotype, allele and haplotype frequency in hypertensive disordercomplicating pregnancy and normal pregnancy group, to explore the associationbetween APM1gene and HDCP and to reveal the role of four SNPs in HDCP patients’abnormal lipid metabolism.The aim of our study is in order to provide valuableresearch information in prevention, diagnosis, treatment and possible future clinicalindividualized therapy and gene therapy of HDCP.Methods: In this study,492subjects of Fujian Han population in total were recruited,consisting of221HDCP patients and271normal pregnant women. We detected thefour single nucleotide polymorphic loci genotypes in Han Chinese women in FujianProvince with the method of polymerase chain reaction-restriction fragment lengthpolymorphism (PCR-RFLP). And we associated with lipids and other clinical data toexplore the genetic correlation between the four SNPs and HDCP. The statisticalanalysis was performed using SHEsis-online software and SPSS17.0.Results:(1) The frequencies of different genotypes of the four SNPs were inagreement with Hardy-Weinberg equilibrium in our study (p>0.05).(2) The genotype and allele frequencies distribution of APM1gene’s four SNPs in theHDCP group①The frequencies of APM1gene-11377C>G’s CC,CG and GG genotypes were 56.6%,36.7%and6.8%, allele frequencies were C74.9%and G25.1%;②The frequencies of APM1gene+45T>G’s TT,TG and GG genotypes were44.5%,46.3%and9.2%, allele frequencies were T67.7%and G32.3%;③The frequencies of APM1gene+712A>G’s AA,AG and GG genotypes were29.7%,54.7%and15.6%, allele frequencies were A57.1%and G42.9%;④The frequencies of APM1gene+4545G>C’s CC,CG and GG genotypes were9.6%,45.2%and45.2%, allele frequencies were C32.2%and G67.8%;(3) The genotype and allele frequencies distribution of APM1gene’s four SNPs in thenormal pregnancy group①The frequencies of APM1gene-11377C>G’s CC,CG and GG genotypes were56.5%,38.4%and5.2%, allele frequencies were C75.6%and G24.4%;②The frequencies of APM1gene+45T>G’s TT,TG and GG genotypes were43.5%,46.1%and10.3%, allele frequencies were T66.6%and G33.4%;③The frequencies of APM1gene+712A>G’s AA,AG and GG genotypes were34.3%,48.3%and17.3%, allele frequencies were A58.5%and G41.5%;④The frequencies of APM1gene+4545G>C’s CC,CG and GG genotypes were10.3%,43.9%and45.8%, allele frequencies were C32.3%and G67.7%;(4) The genotype and allele frequencies of four SNPs of APM1gene, withoutsignificant difference between the HDCP group and normal pregnancy group statisticanalysised by SPSS17.0(p>0.05).(5) The associated analysis between genotypes of APM1gene4SNPs and serum lipidlevels, showed that the+45T>G sites of G carrying genotypes (TG+GG)corresponding to ApoA1, TC and HDL-c levels were significantly higher than that ofTT genotype, the difference was statistically significant (p=0.002,0.045,0.033).(6) We analysis the linkage disequilibrium in all the four SNPs with SHEsis software,demonstrats that the haplotype frequencies consisted by paired SNPs were nosignificantly difference between HDCP group and normal pregnancy group (p>0.05).(7) Multivariate logistic regression analysis showed that systolic blood pressure, BMIindex, TG, ApoA1, ApoB and+4545C/G were into the equation. After adjusting forother confounding factors, serum lipids (p-value of TG, ApoA1and ApoB were0.025, 0.034,0.007; OR were1.592,0.085,0.006) and polymorphic loci+4545G>C weresignificantly associated with occurrence of HDCP (p=0.038, OR=3.767).Conclusion:(1) Four kinds of single nucleotide polymorphisms of APM1gene existin the Han Chinese women in Fujian Province;(2) Strong linkage disequilibriums exist in APM1gene’s four SNPs and+45T>Gcite’s T>G point mutation associated with dyslipidemia increased.(3) APM1gene+4545G>C mutation is a kind of risk in genetics.(4) APM1gene polymorphisms might be related to serum lipid levels of hypertensivedisorder complicating pregnancy, but the exact mechanism ts still unclear, and need tobe further studied. |
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