Research On The Effect Of Compound Guizhu Capsules To Wnt/β-catenin Signal Transduction Pathway On The Mice Of Lewis Lung Carcinoma

Objective:By observe the anti-rumor effects as well as the cell cycle, the expression of β-catenin andcell cycle reglation factor cyclinD1on the Lewis lung cancer, to explore the inhibitionmechanism of Compound Guizhu Capsule (CGZC) from the perspective of signaling patheway,so as to provide an objective experimental foundation for the development and clinicalapplication of CGZC.Methods:Established the mice model of lung transplanted carcinoma through injecting Lewis lungcancer cells in axillary subcutaneous of mice.24hours later, fifty C57BL/6J mice were weighedand divided at random into five groups, which were respectively: model group, cytoxan (CTX)group, high-dose CGZC group, middle-dose CGZC group, and low-dose CGZC group. Therewere10mice in each group, male and female in equal. Then different drugs intervention wasgiven. Model group were perfused0.4ml distilled water into stomachs every day.20mg/kgCTX were injected into abdominal cavity once a day for3days. CGZC diluted with distilledwater according to mice’s weight were perfused into stomachs once a day for10days. The higedose was20g/kg(10times as much as an adult daily dose in clinic), the middle dose was10g/kg(5times as much as an adult daily dose in clinic), and the low dose was5g/kg(2.5timesas much as an adult daily dose in clinic). On the11thday, all the mice were sacrificed afterbeing weighed. The subcutaneous tumors were weighed to calculate inhibitory rate, flowcytometry was used to test the cell cycle and immunohistochemistry method was used to detectthe expression of β-catenin and cyclinD1.Results:The tumor growth was suppressed significantly in CTX group, high-dose CGZC group andmiddle-dose CGZC group(P﹤0.001). CTX group’s anti-rumor effect was higher than Chinesecrude drug groups’(P﹤0.05). Compared with the model group, percentages of the G0/G1phaseof CTX group, high-dose CGZC group and middle-dose CGZC group have remarkablyincreased,and the difference had statistical significance(P﹤0.001), while S and G2/M phasewas the opposite. The percentage of the G0/G1phase of Chinese crude drug groups were lower than CTX group, and the difference also of statistical significance(P﹤0.05). Compared withmodel group, the abnormal expression of β-catenin and cyclinD1were controled in CTX group,high-dose CGZC group and middle-dose CGZC group(P﹤0.05).Conclusions:CGZC can not only significantly inhibit the growth of Lewis lung cancer in mice, but alsorestrain the abnormal expression of β-catenin and cyclinD1. Besides, it can block the cell cyclein G0/G1phase to protect the Lewis lung cancer cells from proliferation. The efficacy ofobstructing the Wnt/β-catenin signaling pathway is probably one of potential mechanism ofCGZC.