| Objective: To extrapolate the possible mechanism of UTI（Ulinastatin） throughanalyzing the changes of gene expression profiles of spleen tissue in septic rats,which is under the regulation of UTI preconditioning, based on DNA microarraytechnology. Methods: Forty-five male Wistar rats were equally divided into shamgroup, sepsis group and UTI group by means of random number table. In UTI groupthe rats were treated with intramuscular injection of UTI(100kUu/kg) an hour beforeCecal ligation and puncture (CLP). In sepsis group and sham group intramuscularbalanced solution (5ml/kg) was given. CLP was used to reproduce septic rat model.The sham group was only experienced a simulated operation not CLP. Geneexpression profile was studied by using RatRef-12Rat gene expression profilemicroarray to detect the changes in gene expression pattern of rat spleen tissue afterCLP. Then using related computer software to screen and analyze the relationshipbetween the Sepsis/UTI group and sham group. At last， analyzing the relationbetween the Sepsis group and UTI group. Results: In22523genes，205differentialgenes were found between sepsis group and sham group, accounting for0.910%.Among them98genes showed up-regulation，with48known functional genes and32genes only shows in this group；107genes showed down-regulation, with64knownfunctional genes and34genes only shows in it.197differential genes were found inUTI group and sham group, accounting for0.875%.Among them114genes showedup-regulation，with35known functional genes and19genes only shows in this group;83genes showed down-regulation, with49known functional genes and19genesonly shows in it. Conclusion: Abnormal expression of genes in the spleen tissue ofrats with sepsis owing to excessive inflammation and immune suppression can bepartly relieved under the UTI preconditioning. To some degree,UTI has a protectiveeffect on spleen at the genetic level. Meanwhile,body may have certain self regulationduring sepsis to act protective effect. |
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