The Effect Of SCLA On Expression Of Survivin Gene Of Human Bladder Cancer T24Cell

Objective:To study the human bladder cancer T24cells growth inhibition and apoptosisinduction function,as well as the influence of protein expression of Survivin,discusses SCLAmechanism of action against human bladder cancer T24cells.Methods:Experimental cells were divided into control group and experimental groups,control group was regular replaced the cell culture medium,without pharmacologicalintervention,the experimental groups according to the drug concentration(0.04g/ml,0.2g/ml,1g/ml,5g/ml)were divided into4groups.After the human bladder cancer T24cells wastreated by different concentrations of SCLA(0g/ml,0.04g/ml,0.2g/ml,1g/ml,5g/ml)for12h、24h、48h and72h respectively,cellular proliferation was determined by the MTTassay；After the human bladder cancer T24cells was treated by different concentrations ofSCLA(0g/ml,0.04g/ml,0.2g/ml,1g/ml,5g/ml)for48h respectively,cell apoptosis rate andthe expression of Survivin protein in cells was detected by the flow cytometry andimmunocytochemistry array.Results:After the human bladder cancer T24cells was treated by different concentrationsof SCLA(0.04g/ml,0.2g/ml,1g/ml,5g/ml)for12h、24h、48h and72h respectively,compared with the control group,were subject to varying degrees of growth inhibition,theapplication of MTT method was measured in control group and the experimental groups OD,calculate proliferation inhibition rate,the results showed human bladder cancer T24cellsproliferation inhibition rate and the drug concentration and action time had a dependencerelation,with the extension of time and the concentration of SCLA increased,the inhibitionrate of human bladder cancer T24cells was more obvious,compared with the control group,the difference was statistically significant(P<0.05),the growth inhibition rate differencesbetween the experimental groups also had statistical significance(P<0.05);The humanbladder cancer T24cells was treated by different concentrations of SCLA(0.04g/ml,0.2g/ml,1g/ml,5g/ml)for48h,using flow cytometry for detection of apoptosis rate were(11.67+2.61)%,(22.40+3.18)%,(38.29+2.30)%,(57.57+3.77)%,the control group apoptosis rate was(1.05+0.34)%,along with the increasing concentration of SCLA,T24bladdercancer apoptosis increases,compared with the control group,the difference was statisticallysignificant(P<0.05),the difference between high concentration and low concentration alsohad statistical significance(P<0.05);After the human bladder cancer T24cells was treatedby different concentrations of SCLA(0.04g/ml,0.2g/ml,1g/ml,5g/ml)for48hrespectively,application of immunocytochemistry assay for detection of Survivin proteinexpression,measurement of gray values were:142.491.67，152.142.66，161.634.44，171.721.17,control group gray value was130.84±4.99,the result showed with the increase ofthe concentration,the gray value was higher and the expression of Survivin protein was lower,compared with the control group,the difference was statistically significant(P<0.05),thedifference between high concentration and low concentration also had statistical significance(P<0.05).Conclusion:①SCLA could inhibit the growth of human bladder cancer T24cells,is timeand concentration dependent;②SCLA could induce bladder cancer T24cell apoptosis,andwith the increase of the concentration of pro-apoptotic effect more obvious;③SCLA coulddownregulate T24bladder cancer cells Survivin protein expression,and with the increasingconcentrations of drug regulatory effect more obvious.