Study On The Chemical Constituents And Anti-inflammation Activity In Vitro Of The Actone Extracts Of Rabdosia Nervosa

Rabdosia nervosa (Hemsi.) C. Y. Wu et H. W. Li., also known as Dayeshezongguan, or Lanhuachaihu or maiyexiangchacai, a perennial herb belonging to rabdosia genus of Labiatae, is widely disturbuted in Shanxi, Henan, Jiangxi, Guangdong, Guangxi, Guizhou, Sichuan and some other provinces of China, and their stems and leaves have been used satisfactorily in the treatment of fever and inflammation along with detoxication, acute infectious heoatitis, snakebite, skin itching, etc.In this thesis, the chemical and pharmacological research progress in the last thirty years on Rabdosia nervosa were was detailedly reviewed.The phytochemical research and anti-inflammation activity in vitro on the acetone extracts of Rabdosia nervosa (Hemsi.)C. Y. Wu et H.W.Li were carried out. In addition the determination method of nodosin, a diterpene of Rabdosia nervosa, was also established.15components were isolated and purified by chromatographic methods, and14components of them were identified on the basis of spectral data (1H-NMR,13C-NMR, DEPT,2D-NMR, ESI-MS, EI-MS) and physicochemical properties. These compounds, including three flavonoids, two diterpenes, three triterpenes, two steroidal and two fatty acids, two other types of components, were elucidated as Apigenin(1), Isorhamnetin(2), pedalitin(3), Nodosin(4), Rabdonervosin J (5), Oleanolic acid(6), Ursolic acid(7),2β,3β-dihydroxyursolic acid (8),3β-hydroxystigmast-5-en-7-one(9),β-daucosterol(10), Hexadecanoic acid(11),(Z)-9-Hexadecenoic acid (12),β-D-Glucose(13), bis (5-formyl-2-furfuryl) ether (14). Among them, compound8,9,12,13,14were isolated from nervosa for the first time, and compounds1,2,3,8,9,12,13,14were isolated from Rabdosia nervosa for the first time15compounds, isolated from the acetone parts of nervosin, were used to find anti-inflammation activity compounds in vitro by LPS-stimulated Raw264.7. Experimental results showed that, compared with the LPS group, Oleanolic acid, Ursolic acid,2β,3β-dihydroxyursolic acid and nodosin (10μg/ml) had significant differences and can significantly reduce the NO content of the LPS-induced Raw264.7. Among them,2β,3β-dihydroxyursolic acid and nodosin, which can significantly reduced the NO content of the LPS-induced Raw264.7was reported for the first time.Develop a HPLC method for the determination of nodosin in the leaves and stems of Rabdosia nervosa. All of reference substances and sample were separated with the mobile phase of methanol:water(37:63)under isocratic elution for40min on a Dikmonsil C18reversed-phase column temperature was25℃. The content of nodosin was0.052mg/g in the stems and0.982mg/g in the leaves, the content in the stems being about one nineteenth of that in leaves. The average recovery of nodosin was99.53%(RSD=1.52%, n=6). The data of the experiment indicated that this method was simple, accurate, specific and suitable for quantitative determination of nodosin in Rabdosia nervosa.