| Research backgroundAlcoholism is an important public health problem in today’s world. It not only seriously affects human health, but also endangers public security and health of future generations. Studies have shown that ethanol has significant reproductive toxicity. Excessive drinking can cause fetal alcohol syndrome for the male during pregnancy, and male alcoholics easily lead to sexual dysfunction and affects fertility and offspring development. The one hand, alcohol through the body blood-testis barrier goes into the human testis which damages the reproductive organs. On the other hand it can also go through blood-brain barrier into brain tissue, and then the free radicals produced by the metabolism directly affect the hypothalamus and pituitary gland, meanwhile, the hypothalamus-pituitary-sex hormone endocrine axis changed, affecting gonadotropin secretion, and the endocrine function of the Sertoli cells. One of two main functions of the testes is to produce sperm. The alcohol is detrimental to the testicle of the male. In the recent years, the report from scholar of the domestic and oversea found the tissue structure of testicle had the different pathological change by the different intake of the alcohol. In the recent years, serum inhibin B levels are paid more and more attention to the spermatogenesis of the testicle and there are many researches about it. Inhibin B was discovered by McCullough in1932and is from the testicle which is synthesized and secreted by the sustenticular cell of the testicle. It has been confirmed that inhibin B is the main marks of function of convoluted tubule of testis. Pierik and others pointed out that the inhibin B level can reflect the function of the testicle and is also the direct the production of the testicle, however, it is not applied to the clinical widely. But, there are no articles of judging the spermatogenesis of the testicle by it combining the serum inhibin B levels with group of pathology typing of the testicle tissue of the drunk mouse.One function of the testicle is to produce the sperm and the spermatogenesis and serum inhibin B,(InhB) synthesis depend on the testicular Sertoli cells. Which serum inhibin B levels reflect the functional status of Sertoli cells, can be used as the evaluation of spermatogenesis. But, there is few of the comprehensive study report from the researcher. The study is the observation about the serum inhibin B levels induced by alcohol and the relationship between the semen quality and testicle structure.ObjectiveBy the rat passive drinking observed in rat serum inhibin B levels change, by changes in the structure of the detection of semen quality (related to parameter changes), and testicular tissue, in order to observe the relationship between the serum inhibition of cytochalasin B and semen quality.Method1. A selection of8weeks old, healthy male SD rats50, weighing150-180g, were numbered and randomly divided into five groups of10each. Control group A, normal diet, distilled water lml/only/day orally; low-dose group B, normal diet, liquor lml/only/day orally; the high dose group C, normal diet, liquor2ml/only/day orally; respectively, continuous administration of13 days (about rat spermatogenic cycle). Low-dose group D, normal diet, liquor1ml/only/day orally; high dose group, normal diet, liquor2ml/only/day orally; continuous administration of26days, respectively (approximately two spermatogenic cycle in rats).2. Before the experiment, the uniform blood collection to exam the serum inhibin B levels.3. By a certain dose, duration, and the law of rats were given passive drinking, the establishment of drinking rat model.4. Again after the experiment to exam the serum inhibin B levels; and to obtain the semen parameters.5. Histological observation about the testicles under the microscope.Result1. Serum inhibin B levels:the experiment before the measurement of serum inhibin B levels were no significant differences between each group and not statistically significant. Short-term (13days) drinking group serum inhibin B levels were significantly lower than the control group and the difference was statistically significant (P＜0.05); long-term (26days) drinking group serum inhibin B levels were significantly lower than the control group and the difference statistically significant (P＜0.05); short-term drinking group with long-term drinking group difference not statistically significant (P>0.05).2. The experimental group (alcohol group) semen analysis showed significantly reduced sperm count, sperm motility, and activities rate compared with normal semen quality of control group. Short-term drinking group and long-term drinking group compared with the control group the difference was statistically significant (P＜0.05); short-term drinking group compared with the long-term drinking group was no significant difference (P>0.05).3. Testicular song seminiferous tubes in the alcohol group (long term) compared with the control group, during which the quality gap widened, the seminiferous tubules of the number of each low magnification from normal controls, seminiferous tubules of the long-term dose group to shrink further thinning, week gap is further widening; groups of spermatogenic cells still exist.Conclusion1. Ethanol makes serum inhibin B levels decline;2. Ethanol can lead to pathological changes in testicular structure with varying degrees;3. Ethanol can reduce semen quality;4. The detection of c serum inhibin B on the clinical assessment of spermatogenesis and semen quality has certain significance. |
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