| Background:Infertility is a global problem, which affect social stability and harmonious family of important factors, With the deepening of the research, a large number of studies that smoking cigarettes is a cause infertility important reason, In the reproductive process of different stages and the period for fertility produce different effect. Cigarette complicated composition. Estimates that4700compounds, Among them more than3000kinds have been recognized, and many matter has been proved to be carcinogenic, teratogenic and cause mutation role. In the smoking process, the mainstream flue gas is active smokers of vulnerable source, Lateral flow flue gas is passive smoking the main hazard sources. Moreover, the latter of inhaled harmful component is the former wants tower above many times. Produce sperm is testicular most important one of two big functions, Smoking on male testicular damage, scholars at home and abroad in recent years were some reports, Think of the testis organizational structure according to the length of time smoking appears different degree of pathological changes. In recent years, with the development of biotechnology, serum statin B in testicular born the role of pure function more and more attention, and done much research. In1932Mccullagh first found statin, Statin B from testis, mainly by testicular synthesis and secretion, support cells, Have now confirmed statin B is testicuiar curved fine fine tube function main marker, therefore statin oppositifolious pure function is of great significance. Pierik etc, points out that serum statin B level reflects the whole testicular tissue function, it is the direct result of testis, Yet its not widely applied in clinic. But serum statin B level and smoking after rat testes histologic subtypes combine to analyze and judge testicular born pure function did not see have reported in the literature.Objective:By making the rat passive smoking, observe the passive smoking rats after testicular tissue microstructure of change. Meanwhile detection rats serum statin B level and semen routine analysis, understand the relation among the three, Through testing the serum statin B level and testicular the degree of damage, In order to get statin B in the evaluation of the testis damaged and born of pure function value of clinical application.Methods:1. Selected healthy male SD rats aged8weeks30and weighing150-180g, one by one number after the statistical methods used to completely randomly divided into smoking group, smoking cessation group and control group.2. Experimental blood test before the unification of the group inhibin B content.3. According to the required dose, duration, rats were given a passive inhalation of cigarette smoke, the establishment of rat model of passive smoking;4. Smoker group10and the control group5ELISA8weeks after the blood serum levels of inhibin B; Profile line of left testicular testosterone HE staining; The left epididymis was cut, suction blow, four-lens cleaning paper filter, the use of semen analysis system to analyze the changes in the indicators rat sperm. Quit smoking group of10control group only with the remaining five after12weeks according to the method of correlation detection.Results:1. Inhibin B test results:Experiment before the measurement of inhibin B in each group showed no difference between content and not statistically significant. Smoking group were significantly lower inhibin B before the experiment and the smoking group was significantly (P<0.01); Smoking cessation group inhibin B were lower than before the experiment, and smoking cessation group significantly (P<0.05);8weeks,12weeks control of inhibin B levels compared with the control group before the experiment was no significant difference was not statistically significant (P>0.05); Quit smoking group compared with no significant difference (P>0.05); Smoking group compared with the control group8weeks, significant difference, statistically significant (P<0.01); Smoking cessation group and12week control group was significantly (P<0.05);8weeks and12weeks the control group no significant difference (P>0.05).2. Smoking and smoking cessation group structure of testicular tissue pathological changes of various degrees, while the control group was normal.3. Smoking and smoking cessation group semen analysis showed significantly reduced sperm count, sperm motility, lower activity rates,8and12weeks are in the normal control group. Sperm concentration, sperm motility quit smoking group compared with no significant difference (P>0.05); Smoking group and8weeks, significantly lower than the control group, the difference was significant, with statistical significance (P<0.01); Smoking cessation group and12week control group was significantly (P<0.05);8weeks and12weeks compared with the control group no significant difference (P>0.05).Conclusion:1. Passive smoking has caused testicular structure in different degree pathological changes,2. Passive smoking make serum statin B level drops,3. Passive smoking has caused testicular born pure function is reduced,4. Serum statin B detection of evaluation testicular born pure function of great significance. |
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