Study On The Relationship Between Brg1and Laryngocarcinoma

Brg1is a component of the evolutionarily conserved SWI/SNF chromatin remodelingcomplex and a tumor suppressor gene discovered in recent years. Brg1plays an importantrole in regulating the cell cycle, development and differentiation, DNA repaired and cancerdevelopment. Brg1can activate or repress transcription by interacting with cytokines as RB,p53, CD44, BRCA-1and so on. Study shows that Brg1is mutated and deleted in thecarcinoma cell lines of lung, breast, prostate and pancreas. This study explored therelationship between Brg1gene and laryngocarcinoma by PCR-RFLP andimmunohistochemical methods. Compared with98sporic laryngocarcinoma and100controls found that the allele frequencies in rs60448955, rs12609589, rs75904516of Brg1had no remarkable difference between case and control groups(P＞0.05); the G, C allelefrequencies in rs73013167had remarkable difference between case and control groups(P＜0.05), rs73013167might have a relationship with laryngocarcinoma. To detect theexpression of Brg1in20cases of laryngocarcinoma and10cases of normal tissue by usingimmunohistochemical method, it is found that the expression of Brg1in normal tissue waspositive, the positive rate was90%; the expression of Brg1in laryngocarcinoma was lower,the positive rate was35%, the two groups had remarkable in positive rate(P＜0.05).However, the positive expression of Brg1gradually reduced in well, moderate and poor oflaryngocarcinoma; the positive rate of lymph node metastasis was lower than no lymph nodemetastasis. Brg1plays an important role in the development of laryngocarcinoma,maintaining normal morphology and function of laryngeal mucosa. The report for Brg1andlaryngocarcinoma was rare, this study demonstrated the relationship between Brg1andlaryngocarcinoma by molecular biology and histology, and founded the further explorationthe molecular mechanism of laryngeal carcinoma, and provided a new field for clinicaldiagnosis and treatment.